Agaricus bisporus Lectin (ABA/ABL) - FITC (Fluorescein)

Cat# 20810008-1

Size : 2mg

Brand : BioWorld


Description

Affinity-pured Agaricus bisporus agglutinin (ABA) (SKU 21510154) is a tetramer composed of two to four isolectins. ABA has two distinct carbohydrate binding sites, one for galactose-ß-1,3-N-acetylgalactosamine and another for galactose-ß-1,3-N-acetylglucosamine. Agaricus bisporus has an anti-proliferative effect on cancerous cells. ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins, thus making ABA an inhibitor of its nuclear import of signal-dependent proteins.
Agaricus bisporus Lectin (ABA/ABL) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications.

Abbreviation: ABA/ABL
Material Source: Agaricus bisporus Mushroom
Conjugate: FITC (Fluorescein)
Concentration: 2 mg/mL
Shelf Life: 1 year
Blood Group Specificity: Non-specific
Preferred Sugar Specificity: Galactoseβ1-3N-Acetylgalactosamine
Inhibiting or Eluting Sugar: Fetuin
Fluorescence: Green
Divalent Ions: None Required
Excitation: 495 nm
Emission: 515 nm
Mitogenic Activity: No
Lyophilized or Liquid: Liquid
Storage Temperature: 2-8°C
Hazardous Shipping: Non-hazardous

Application

Immunofluorescence, Glycobiology

References

Batterbury, M., Tebbs, C. A., Rhodes, J. M., & Grierson, I. (2002). Agaricus bisporus (edible mushroom lectin) inhibits ocular fibroblast proliferation and collagen lattice contraction. Experimental eye research, 74(3), “370. https://doi.org/10.1006/exer.2001.1133

Kent, D., Sheridan, C. M., Tomkinson, H. A., White, S. J., Hiscott, P., Yu, L., & Grierson, I. (2003). Edible mushroom (Agaricus bisporus) lectin inhibits human retinal pigment epithelial cell proliferation in vitro. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 11(4), “291. https://doi.org/10.1046/j.1524-475x.2003.11408.x

Properties

Shelf life

2 years

Storage Temperature

-20°C

ECCN #

EAR99

Hazmat Ship

Non-Hazardous

Purity

Affinity Purified

Appearance color

Clear, Light Orange

Appearance form

Liquid

Abbreviation (Lectins Only):

ABA/ABL

Source

Mushroom

Blood Group Specificity

A,B - HighO - MidNeuraminidase - Low

Carbohydrate Specificity

Galactoseβ1-3N-Acetylgalactosamine

Storage Buffer

1x PBS w/ 0.02% Sodium Azide

Conjugate/Tag/Matrix

FITC (Fluorescein)

Inhibitory Carbohydrate

Fetuin

Fluorescence

Green

Divalent Ions

None Required

Excitation

495 nm

Mitogenic Activity

No

Emission

515 nm

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