3D scaffold for hepatocyte - Black plates (fluorescence)
BIOMIMESYS® Liver hydroscaffold is made of RGDS and galactosamine-grafted Hyaluronic acid, Adipic acid dihydrazide crosslinker and extracellular matrix (ECM) proteins (collagen type I and collagen type IV) to mimic liver-ECM composition. The SEM image shows the biomimetic structure of the BIOMEMISYS® Liver hydroscaffold with collagen chains.
A factor which greatly determines cell activity and viability is the extracellular matrix (ECM). Liver cells are organized and maintained by a complex extracellular microarchitecture. ECM compounds play a tremendous role in liver function regulation. Collagens, glycoproteins and glycosaminoglycans (GAG) constitute the majority of the ECM liver; also play important roles in cell physiology.
Spheroid: Viability & Growth: Increase Culture Time
Primary human hepatocytes and hepatocarcinoma cell line, as HepG2, remain viable for longer time in culture compared to 2D. Furthermore, primary human hepatocytes keep their metabolic activity for longer time in culture compared to 2D, even in sandwich culture.
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