hot-start DNA polymérases

hot-start DNA polymérases




Hot start DNA polymerase is a modified form of DNA polymerase which avoids a non-specific amplification of DNA by inactivation of the taq polymerase at low temperatures. The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. In Hot start PCR, specific antibodies are used to block the Taq-polymerase at lower temperature. An initial step at 95℃ is required for denaturing the antibodies linked to the active center of the enzyme. The anti-Taq antibodies reduce the Taq polymerase activity below 72℃, the optimal temperature at which the enzyme extends the primers. When the specific antibodies detach from Taq-polymerase, the amplification proceeds with greater specificity.

Classical DNA polymerase is active at room temperature and to a lesser degree, even on ice. In some instances, when all the reaction components are put together, nonspecific primer annealing can occur due to these low temperatures. This nonspecific annealed primer can then be extended by the DNA polymerase, generating nonspecific products and lowering product yields.

Hot Start PCR significantly reduces nonspecific priming, the formation of primer dimers, and often, increases product yields

We offer a wide range of Hot-Start Taq DNA polymerase for use in PCR. Please find below a selection with main features :

Name HS Taq DNA polymerase AceTaq DNA polymerase TransStart taq DNA polymerase
Product code NB-03-0103 to NB-03-0106 P401 AP141
Type Taq polymerase Taq polymerase Taq Polymerase
Speed 0.9-1.2 kb/min 1 kb/min 1-2 kb/min
Amplicon size < 5kb < 5kb < 15 kb
5'-3' exonuclease activity Yes Yes Yes
3'-5' exonuclease activity No Yes No


List of the products

Cat#
Description
Size
Price
NB-15-1547
 2 x 250 U 
NB-15-1546
 250 U 
GEN-0049-1
 1 KU 
GEN-0049-250
 250 U 
AP141-03
 6 x 500 u 
P401-d1
 250 U 
P401-d2
 1000 U 
P401-d3
 3000 U 
L7052001
 1 KU 
L7052250
 250 U 
E00049-1000
 1 KU 
E00049-250
 250 U 
MBS136047-1000U
 1000 Units 
MBS136047-500U
 500 Units