hot-start DNA polymérases
Hot start DNA polymerase is a modified form of DNA polymerase which avoids a non-specific amplification of DNA by inactivation of the taq polymerase at low temperatures. The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. In Hot start PCR, specific antibodies are used to block the Taq-polymerase at lower temperature. An initial step at 95℃ is required for denaturing the antibodies linked to the active center of the enzyme. The anti-Taq antibodies reduce the Taq polymerase activity below 72℃, the optimal temperature at which the enzyme extends the primers. When the specific antibodies detach from Taq-polymerase, the amplification proceeds with greater specificity.
Classical DNA polymerase is active at room temperature and to a lesser degree, even on ice. In some instances, when all the reaction components are put together, nonspecific primer annealing can occur due to these low temperatures. This nonspecific annealed primer can then be extended by the DNA polymerase, generating nonspecific products and lowering product yields.
Hot Start PCR significantly reduces nonspecific priming, the formation of primer dimers, and often, increases product yields
We offer a wide range of Hot-Start Taq DNA polymerase for use in PCR. Please find below a selection with main features :
|Name||HS Taq DNA polymerase||AceTaq DNA polymerase||TransStart taq DNA polymerase|
|Product code||NB-03-0103 to NB-03-0106||P401||AP141|
|Type||Taq polymerase||Taq polymerase||Taq Polymerase|
|Speed||0.9-1.2 kb/min||1 kb/min||1-2 kb/min|
|Amplicon size||< 5kb||< 5kb||< 15 kb|
|5'-3' exonuclease activity||Yes||Yes||Yes|
|3'-5' exonuclease activity||No||Yes||No|