HRP Chromogen for IHC - DAB
DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).
Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling
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- Unconjugated 864
- HRP 46
- Biotin 10
- APC 5
- Alexa Fluor 350 5
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- Cy3 5
- Cy5 5
- Cy5.5 5
- Cy7 5
- FITC 5
- PE 5
- human 366
- e. coli 70
- cow 40
- camel 16
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- plant 5
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- yeast 3
- monosan dab high contrast 2
- virus 2
- 1 1
- monosan plus (hrp) broad spectrum (dab)kit 1
- human 241
- yeast 206
- e. coli 158
- 36
- horse 12
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- dog 10
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- sheep 10
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- bird 5
- cat 1
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- Biochemicals 1262
- Protein/peptide 1116
- Inhibitor/Antagonist/Agonist 473
- ELISA/ELISPOT 360
- Primary antibody 278
- kit 104
- Buffers and reagents 92
- Probe 56
- Other products 22
- cDNA Clone 19
- Secondary antibody 14
- RNAi 10
- Lysate 2
- CRISPR/Cas9 1
- Paraffin tissue section 1
- Primer 1
- ELISA 368
- IHC 81
- WB 32
- IP 8
- IF 6
- ICC 5
- 0.078 ng/mL 3
- 0.1 ng/mL 2
- 0.116 ng/ml 1
- PCR 1
- Polyclonal 16
- 10.1F8 5
- 13.1G11 5
- 13.2G8 5
- 13.6D9 5
- 13.7H4 5
- 13B4.E10 5
- 14.2H9 5
- 26.7C4 5
- 3G8 5
- 4A8 5
- 6.1H3.D9.C6 5
- 6.1H8.F9.G6 5
- 6.7A2.A4.D3 5
- 6.8E7.A7.A1 5
- 8C7 5
- 8G1 5
- 8H6 5
- 9.21C1.G1.F1.A5 5
- 9.22E1.F5.H10 5
- 9.24E10.F3.H12 5
- 12H6.D3 4
- 13.7E2 4
- 13.8E5 4
- 14F10.H9 4
- 1A6 4
- 2E4 4
- 4B9 4
- 5A11 4
- 5A2 4
- 6.2D2.E1.D11 4
- 6.8A2.B8.A11 4
- 8H12 4
- IgG.2A5 4
- IgG.2E9 4
- 3
- Monoclonal 2
- 001.11D9 1
- 001.11F2 1
- 001.6C2 1
- 001.7B11 1
- 13.7E2. 1
- 13.8E5. 1
- 6. 8A2.B8.A11 1
- E1A 1
- E1B 1
- Recombinant 1
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