IHC buffers - Antigen retrieval

IHC buffers - Antigen retrieval


Most formalin-fixed tissues require an antigen retrieval step before immunohistochemical staining. Although fixation is essential for the preservation of tissue morphology, this process can also have a negative impact on IHC/ICC detection. Fixation can alter protein biochemistry such that the epitope of interest is masked and can no longer bind to the primary antibody. Methylene bridges formed during fixation cross-link proteins and mask antigenic sites. Antigen retrieval methods break these methylene bridges and expose antigenic sites, allowing antibodies to bind. The two methods for antigen retrieval are heat induced epitope retrieval (HIER) and enzymatic retrieval.

We offer a range of HIER Retrieval Buffers with different pH and packaging (ready-to-use or concentrated) to find the one that best suits your protocol.


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Description
Cond.
Price Bef. VAT
NB-23-00178-4
 50mL 
NB-23-00178-3
 125mL 
NB-23-00174
 100mL 
NB-23-00178-1
 1000mL 
NB-23-00172
 1000mL 
NB-23-00178-2
 1000mL 
NB-47-02328-250ML
 250ml 
NB-23-00173
 100mL 
NB-23-00175
 100mL 
NB-23-00176
 100mL 
NB-23-00177
 1000mL 
42020004-1
 250mL 
orb1787667-250ml
 250ml 
40121000-1
 500mL