Datura stramonium Lectin (DSA/DSL) - Pure

Cat# 21510038-2

Size : 5mg

Brand : BioWorld


Description

Datura stramonium agglutinin or lectin (DSA/DSL) is purified by affinity chromatography. Seeds of Datura stramonium contain at least three different carbohydrate-binding proteins, and the most prominent lectin is a dimeric glycoprotein composed of two nonidentical subunits that contains 40% carbohydrate. It binds well to N-acetylglucosamine, oligomers, and branched pentasaccharide, including two N-acetylglucosamine disaccharides linked to mannose (?-1,6) and (?-1,2), which is known to be the most potent inhibitor of agglutination. DSL prefers binding to (GlcNAc)2-4 and elutes with the sugar chitin hydrolysate. DSL binds well in the acidic pH range and its affinity decreases above pH 8.0. This agglutinin has blood group A, B, and O specificity. Studies have shown that DSA can act as a biomarker for neoplastic urotherial cells.

Properties

Shelf life

2 years

Storage Temperature

-20°C

ECCN #

EAR99

Hazmat Ship

Non-Hazardous

Purity

Affinity purified

Appearance form

Lyophilized Powder

Molecular Weight

86 kDa

Solubility (@ RT)

Soluble in phosphate buffered saline, pH 6.8

Abbreviation (Lectins Only):

DSA/DSL

Source

Jimson weed

Blood Group Specificity

A, B, O

Carbohydrate Specificity

(N-Acetylglucosamine)2-4

Conjugate/Tag/Matrix

None

Inhibitory Carbohydrate

Chitin Hydrolysate

Divalent Ions

None Required

Cancer Biomarker

N-cadherin on cancerous bladder cell tissue

Mitogenic Activity

Yes

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