Datura stramonium Lectin (DSA/DSL) - Pure
Cat# 21510038-2
Size : 5mg
Brand : BioWorld
Description
Datura stramonium agglutinin or lectin (DSA/DSL) is purified by affinity chromatography. Seeds of Datura stramonium contain at least three different carbohydrate-binding proteins, and the most prominent lectin is a dimeric glycoprotein composed of two nonidentical subunits that contains 40% carbohydrate. It binds well to N-acetylglucosamine, oligomers, and branched pentasaccharide, including two N-acetylglucosamine disaccharides linked to mannose (?-1,6) and (?-1,2), which is known to be the most potent inhibitor of agglutination. DSL prefers binding to (GlcNAc)2-4 and elutes with the sugar chitin hydrolysate. DSL binds well in the acidic pH range and its affinity decreases above pH 8.0. This agglutinin has blood group A, B, and O specificity. Studies have shown that DSA can act as a biomarker for neoplastic urotherial cells.
Properties
Shelf life
2 years
Storage Temperature
-20°C
ECCN #
EAR99
Hazmat Ship
Non-Hazardous
Purity
Affinity purified
Appearance form
Lyophilized Powder
Molecular Weight
86 kDa
Solubility (@ RT)
Soluble in phosphate buffered saline, pH 6.8
Abbreviation (Lectins Only):
DSA/DSL
Source
Jimson weed
Blood Group Specificity
A, B, O
Carbohydrate Specificity
(N-Acetylglucosamine)2-4
Conjugate/Tag/Matrix
None
Inhibitory Carbohydrate
Chitin Hydrolysate
Divalent Ions
None Required
Cancer Biomarker
N-cadherin on cancerous bladder cell tissue
Mitogenic Activity
Yes