TNFα is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNFα secreted by the macrophage causes the blood to clot which provides containment of the infection. TNFα binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

The biological activity of Human TNFα was measured in a cytotoxicity assay using the TNF susceptible murine L929 cell line in the presence of the metabolic inhibitor actinomycin D (Matthews, N. and M.L. Neale in Lymphokines and Interferons, a Practical Approach, 1987, M.J. Clemens, A.G. Morris and A.J.H. Gearing, eds., IRL Press, p. 221). The expected ED₅₀ for this effect is typically 25 100 pg/ml.

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Met & Val77

Lyophilized

The predicted molecular weight of Recombinant Human TNFα is Mr 17.5 kDa. However, the actual molecular weight as observed by migration on SDSPAGE is Mr 17 kDa.

17.5

This recombinant protein was lyophilized from a 0.2 μm filtered solution in deionized water.

The lyophilized protein should be stored desiccated at 20°C. The reconstituted protein can be stored for at least one week at 4°C. For longterm storage of the reconstituted protein, aliquot into working volumes and store at 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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