LDH Cytotoxicity Assay Kit (Fluorometric)
Cat# K2267-200
Size : 200T
Brand : APExBIO Technology
LDH Cytotoxicity Assay Kit (Fluorometric)
LDH Cytotoxicity Assay Kit (Fluorometric) is a kit that assesses cytotoxicity by measuring the activity of lactate dehydrogenase (LDH) released into culture media. LDH is a stable enzyme found in all cells and is released from the cell into the medium when cells are damaged or apoptosis. LDH catalyzes the conversion of lactate to pyruvate, and this catalytic process reduces NAD+ to NADH. The specific substrates provided in this kit can react with the resulting NADH to produce a red fluorescent product. The fluorescence intensity was positively correlated with LDH activity. Therefore, by measuring the fluorescence intensity, it is possible to indirectly assess the degree of cell damage or death.
Compared with the traditional 51Cr cytotoxicity test, this kit contains no radioactive substances, which is simpler and safer to use. Moreover, the detection effect of this kit is consistent with the 51Cr test, and the results are reliable. The main difference between this kit and the LDH Cytotoxicity Assay Kit (K2228) is the detection method, this kit detects fluorescence, and K2228 detects absorbance, which can be selected according to specific needs.
Figure 1: LDH release as measured by this kit
| Components | K2267-200T | K2267-1000T |
| Substrate Mix | 1 vial | 5 x 1 vial |
| Reporter Mix | 12 mL | 5 x 12 mL |
| Lysis Buffer (10X) | 2.5 mL | 12 mL |
| Stop Solution | 12 mL | 60 mL |
| LDH Positive Control | 6 μL | 30 μL |
| Store the kit at -20°C, stable for 1 year. Substrate Mix should be stored away from light. | ||