Reagents and instruments for immunology, cell biology and molecular biology.

Anti-SUZ12 (Suppressor of Zeste 12 Protein Homolog, CHET9, JJAZF1, KIAA0160, LOC23512) (APC) Monoclonal Antibody

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Cat# S8995-01-APC-100ul

Size : 100ul

Brand : US Biological

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S8995-01-APC SUZ12 (Suppressor of Zeste 12 Protein Homolog, CHET9, JJAZF1, KIAA0160, LOC23512) (APC)

Clone Type

Polyclonal

Host

mouse

Source

human

Swiss Prot

Q15022

Isotype

IgG1,k

Grade

Affinity Purified

Applications

FLISA WB

Crossreactivity

Accession #

NM_015355

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

Applications:
Suitable for use in FLISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 Lysates from HeLa cells were resolved by electrophoresis, transferred to PVDF and probed with S8995-01. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection.
Optimal dilutions to be determined by the researcher.

Recommended secondary antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

Recommended Positive Antigen Control:
H1838: HeLa nuclear extract.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1,k|Clone No: 7L26 (3C1.2)|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A affinity chromatography.|Immunogen: Recombinant protein corresponding to full length human SUZ12. |Specificity: Recognizes human SUZ12 at 95kD.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Recombinant protein corresponding to full length human SUZ12.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

Purity

Purified by Protein A affinity chromatography.

Specificity

Recognizes human SUZ12 at 95kD.

References

1. Nucci, Marisa R. et al. (2007). Molecular analysis of the JAZF1-JJAZ1 gene fusion by RT-PCR and |fluorescence in situ hybridization in endometrial stromal neoplasms. Am J Surg Pathol 31: 65-70 |2. Furuno, Kenji. et al. (2006). Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro. Biochem Biophys Res Commun 345: 1051-8. 3. Lee, Tong Ihn et al. (2006). Control of developmental regulators by Polycomb in human embryonic stem cells. Cell 125: 301-13. 4. Huang, Hsuan-Ying et al. (2004). Molecular detection of JAZF1-JJAZ1 gene fusion in endometrial stromal neoplasms with classic and variant histology: evidence for genetic heterogeneity. Am J Surg Pathol 28: 224-32.