PtX™ Rabbit Anti-Human Chorionic Gonadotropin Recombinant Antibody
Rabbit Anti-hCG
Recombinant rabbit monoclonal antibody against Human Chorionic Gonadotrophin protein. This product is produced in Nicotiana benthamiana plants for use in ELISA applications.
100 µg | Catalogue Number: CBT_A0043
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Human Chorionic Gonadotrophin (hCG) is a glycoprotein hormone that is primarily produced by the placenta during pregnancy. It is composed of an alpha and beta subunit, where the alpha subunit shares homology to other glycoprotein hormones such as follicle-stimulating hormone (FSH), luteinizing hormone (LH), and thyroid-stimulating hormone (TSH). The beta subunit, on the other hand, is unique to hCG and is responsible for its biological activity, that includes promoting development and maintenance of the corpus luteum, which produces progesterone to support pregnancy. Additionally, hCG is commonly used as a marker for pregnancy and is also used in the treatment of certain medical conditions such as infertility and testicular cancer. The high specificity of our human anti-chorionic gonadotrophin antibody to the beta subunit of native form hCG ensures accurate and reliable results. It is suitable for ELISA application and designed to provide researchers with a powerful tool for studying the biology and function of hCG in fertility health and disease.
Additional information
| Available Unit Size | 0.1mg, 1mg, 10mg, 100mg, 200mg |
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Key Benefits
This product is a recombinant monoclonal antibody that offers several benefits including the following:
- Batch-to-batch consistency.
- This product is plant-made, no animals or animal products are used in the manufacture of this protein.
- BSA and Azide free.
For more information on plant-made, recombinant monoclonal antibodies click here.
Datasheet
| Product Name | PtX™ Rabbit Anti-Human Chorionic Gonadotropin Recombinant Antibody |
| Catalogue Number | CBT_A0043 |
| Expression Host | Nicotiana benthamiana plants |
| Clonality | Monoclonal, recombinant |
| Species and Isotype | Rabbit IgG1 |
| Tag | None |
| Reporter Protein | None |
| Description | This product is a full-length Rabbit IgG1 recombinant antibody specific to Native Human hCG protein. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration. |
| Target Name | Native Human hCG protein |
| Verified Applications | ELISA |
| Dilution Range | ELISA (1: 3 000 – 1: 256 000) |
| Tested Species Reactivity | Human |
| Concentration | 1.0 mg/ml |
| Form | Liquid |
| Colour | Clear |
| Preparation | Ready to use |
| Storage | Short-term (up to one week): 2 – 8 °C Long term: Aliquot and store at – 20 °C Store immediately. Aliquot and avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1 M Phosphate Buffered Saline, pH 7.4 Preservative: none |
| Purification Notes | This product was purified using Protein A- affinity chromatography. |
| Purity | ≥ 95 % as determined by SDS-PAGE. |
| General Notes | If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing . |
Images
Figure 1. ELISA Dose Response curve using PtX™ Rabbit Anti-hCG antibody from 0.3 – 0.0014 ng/µl to detect 25 ng Native Human hCG protein. Experiments were performed in triplicate, with error bars representing standard deviation (SD).
Figure 2. ELISA Dose Response curve to detect 25 ng Native Human hCG protein using PtX™ Rabbit Anti-hCG antibody from 0.3 – 0.0014 ng/µl stored at -20°C and shipping conditions for two weeks. At shipping the product is placed under conditions that simulate real time shipping conditions. Experiments were performed in triplicate, with error bars representing standard deviation (SD).
PtX™ | Plant-Based Expression
The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.
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