Lotus tetragonolobus (LTL)

Cat# L3444-50-5mg

Size : 5mg

Brand : US Biological



Grade
Affinity Purified

Shipping Temp
Blue Ice

Storage Temp
-20°C

Asparagus Pea
Affinity purified Lotus tetragonolobus lectin (LTL) is isolated from lotus seeds and is composed of 240 amino acid residues. LTL is a glycoprotein consists of four subunits. This lectin has different isoelectric points of pH 7.3, 7.6, 7.9, 8.2 and a carbohydrate specificity toward alpha-fucose, it is most inhibited by L-fucose. LTL is a fucose-binding anti-H(O) lectin. Transitional cell carcinoma of the human urinary bladder tends to metastasize when it strongly expresses binding sites for LTL.
Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.

Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Carbohydrate Specificity: L-Fucose

Inhibitory Carbohydrate: alpha L-Fucose

Activity:
Less than 10ug/ml will agglutinate neuraminidase treated type O human erythrocytes.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Source
Asparagus Pea

Purity
Affinity Purified

Concentration
~1mg/ml (after reconstitution)

Form
Supplied as a lyophilized powder from PBS, pH 7.3. Reconstitute with 1ml sterile PBS.


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.