Reagents and instruments for immunology, cell biology and molecular biology.
 
> Anti-CD45R (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (FITC) Monoclonal Antibody

Anti-CD45R (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (FITC) Monoclonal Antibody

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Cat# C2400-27F-FITC-100ul

Size : 100ul

Brand : US Biological

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C2400-27F-FITC CD45R (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (FITC)

Clone Type
Polyclonal
Host
mouse
Source
sheep
Isotype
IgG1
Grade
Affinity Purified
Applications
FC IHC IP
Crossreactivity
Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD45R cell surface antigen, is a 220kD glycoprotein, which is expressed by all B lymphocytes and a subset of T lymphocytes. In immunohistochemical staining the antibody labels all lymphocytes within the B cell follicles as well as scattered cells in interfollicular areas.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 20.96|Host: mouse|Source: sheep|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Protein G affinity chromatography.|Immunogen: Ovine thymocytes.|Specificity: Recognizes sheep CD45R cell surface antigen.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Ovine thymocytes.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes sheep CD45R cell surface antigen.
References
1. Mackay, C. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset. Cell. Immunol. 110: 46 -55. 2. Sainte Marie, G. et al. (1962) A paraffin embedding technique for studies employing immunofluorescence. J. Histochem. Cytochem.