Triticum vulgaris (WGA) (FITC)

Triticum vulgaris Lectin (WGA) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. Fluorescein conjugates display a high rate of photobleaching, signal is sensitive to pH changes, broad fluorescence emission spectrum, and fluorescence quenching on conjugation to biopolymers.

Source:
Wheat

Blood Specificity:
A, B, O

Sugar Specificity:
N-Acetylglucosamine

Inhibitory Carbohydrate:
Chitin Hydrolysate or N-Acetylglucosamine w/acid or salt

Fluorescence:
Green

Divalent Ions:
Ca++

Excitation:
495nm

Emission:
515nm

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.