Ammonia (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH3 are very desirable. Ammonia/ammonium assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ammonium and forms a fluorescent product. The fluorescence intensity (lex/em=360/450nm) is proportional to the ammonia concentration in the sample.

Key Features:
Fast and sensitive: Linear detection range of 0.012-1mM ammonia.
Convenient and high-throughput: Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Applications:
Ammonia/ammonium determination in biological (e.g. urine) and environmental samples.

Kit Components:
A1383-56A: Reagent A, 1x1ml
A1383-56B: Reagent B, 1x1ml
A1383-56C: Assay Buffer, 1x20ml
A1383-56D: Standard (NH4Cl), 1x400ul

Storage and Stability:
Store kit at -20°C. Stable for 6 months after receipt.