Reagents and instruments for immunology, cell biology and molecular biology.

Anti-NKp46, Natural Cytotoxicity Receptor (NCR NKp46, CD335, Lymphocyte Antigen 94, Ly94, Natural Cytotoxicity Triggering Receptor 1, NCR1, NCT1, Natural Killer Cell Activating Receptor NKp46, NK Cell Activating Receptor) (FITC) Monoclonal Antibody

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Cat# N2835-01D-FITC-100ul

Size : 100ul

Brand : US Biological

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N2835-01D-FITC NKp46, Natural Cytotoxicity Receptor (NCR NKp46, CD335, Lymphocyte Antigen 94, Ly94, Natural Cytotoxicity Triggering Receptor 1, NCR1, NCT1, Natural Killer Cell Activating Receptor NKp46, NK Cell Activating Receptor) (FITC)

Clone Type

Polyclonal

Host

mouse

Source

bovine

Isotype

IgG1

Grade

Affinity Purified

Applications

FC IF WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor. CD335 is expressed by human natural killer cells (4). The bovine homolog is expressed on bovine NK cells (2) and no expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.||Applications:|Suitable for use in Flow Cytometry, Immunofluorescence and Western Blot. Other applications not tested.||Recommended Dilution:|Flow Cytometry: 10ul labels 10e6 cells in 100ul|Optimal dilutions to be determined by the researcher||Hybridoma: |NS-0 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: 10B1431 (AKS1)|Host: mouse|Source: bovine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant. |Immunogen: Fusion protein consisting of the extracellular region of bovine CD335.|Specificity: Recognizes bovine CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1. Species Crossreactivity: American bison||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Fusion protein consisting of the extracellular region of bovine CD335.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant.

Specificity

Recognizes bovine CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1. Species Crossreactivity: American bison

References

1. Storset, A.K. et al. (2004) NKp46 defines a subset of bovine leukocytes with natural killer cell characteristics. Eur J Immunol. 34 (3): 669-76. 2. Kulberg, S. et al. (2004) Reference values for relative numbers of natural killer cells in cattle blood. Dev Comp Immunol. 28 (9): 941-8. 3. Van Rhijn, I. et al. (2007) Massive, sustained gamma/delta T cell migration from the bovine skin in vivo. J Leukoc Biol. 81: 968-73. 4. Toka, F.N. et al. (2011) Rapid and Transient Activation of gamma/delta T Cells to IFN-gamma Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection. J Immunol. 186: 4853-61. 5. Bastos, R.G. et al. (2008) Bovine NK cells acquire cytotoxic activity and produce IFN-γ after stimulation by Mycobacterium bovis BCG- or Babesia bovis-exposed splenic dendritic cells. Vet Immunol Immunopathol. 124: 302-12. 6. Boysen, P. et al. (2006) Bovine CD2-/NKp46+ cells are fully functional natural killer cells with a high activation status. BMC Immunol. 7:10. 7. Elhmouzi-Younes, J. et al. (2009) Bovine neonate natural killer cells are fully functional and highly responsive to interleukin-15 and to NKp46 receptor stimulation. Vet Res. 40: 54. 8. Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells. Vet Res. 41:4. 9. Hoek, A. et al. (2009) Subpopulations of bovine WC1(+) gamma/delta T cells rather than CD4(+)CD25(high) Foxp3(+) T cells act as immune regulatory cells ex vivo. Vet Res. 40:6. 10. Zuerner, R.L. et al. (2011) A Leptospira borgpetersenii Serovar Hardjo Vaccine Induces a Th1 Response, Activates NK Cells, and Reduces Renal Colonization. Clin Vaccine Immunol. 18: 684-91. 11. Nelson, D.D. et al. (2010) CD8(+)/perforin(+)/WC1(- ) gamma/delta T cells, not CD8(+) alpha/beta T cells, infiltrate vasculitis lesions of American bison (Bison bison) with experimental sheep-associated malignant catarrhal fever. Vet Immunol Immunopathol. 136: 284-91. 12. Banos, G. et al. (2013) Identification of immune traits correlated with dairy cow health, reproduction and productivity. PLoS One. 8: e65766. 13. Ramstead, A.G. et al. (2015) Aging influences the response of T cells to stimulation by the ellagitannin, oenothein B. Int Immunopharmacol. 26 (2): 367-77. 14. Boysen, P. et al. (2008) Natural killer cells in lymph nodes of healthy calves express CD16 and show both cytotoxic and cytokine-producing properties. Dev Comp Immunol. 32 (7): 773-83. 15. González-Cano, P. et al. (2014) Two functionally distinct myeloid dendritic cell subpopulations are present in bovine blood. Dev Comp Immunol. 44 (2): 378-88. 16. Di Palma, S. et al. (2012) Comparative spatiotemporal analysis of the intrathecal immune response in natural listeric rhombencephalitis of cattle and small ruminants. Comp Immunol Microbiol Infect Dis. 35 (5): 429-41. 17. Pirson, C. et al. (2015) Highly purified mycobacterial phosphatidylinositol mannosides drive cell-mediated responses and activate NKT cells in cattle. Clin Vaccine Immunol. 22 (2): 178-84. 18. Schneider, D.A. et al. (2011) Dynamics of bovine spleen cell populations during the acute response to Babesia bovis infection: an immunohistological study. Parasite Immunol. 33 (1): 34-44. 19. Van Meulder F et al. (2015) Analysis of the protective immune response following intramuscular vaccination of calves against the intestinal parasite Cooperia oncophora. Int J Parasitol. 45 (9-10): 637-46. 20. Patch, J.R. et al. (2014) Infection with foot-and-mouth disease virus (FMDV) induces a natural killer (NK) cell response in cattle that is lacking following vaccination. Comp Immunol Microbiol Infect Dis. 37 (4): 249-57. 21. Hedges, J.F. et al. (2015) Amphotericin B stimulates gamma delta T and NK cells, and enhances protection from Salmonella infection. Innate Immun. pii: 1753425914567692. 22. Allan AJ et al. (2015) Cattle NK Cell Heterogeneity and the Influence of MHC Class I. J Immunol. pii: 1500227. 1. Sivori, S. et al. (1997) p46, a novel natural killer cell-specific surface molecule that mediates cell activation. J Exp Med. 186 (7): 1129-36. 2. Storset, A.K. et al. (2003) Natural killer cell receptors in cattle: a bovine killer cell immunoglobulin-like receptor multigene family contains members with divergent signaling motifs. Eur J Immunol. 33 (4): 980-90.