Reagents and instruments for immunology, cell biology and molecular biology.

Anti-Phospho-PDCD4-Ser67 antibody (10-90)

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Cat# NB-22-0547-200ul

Size : 200µg

Brand : Neo Biotech

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of lysates from HUVEC cells, K562 cells and COS-7 cells, using PDCD4 (Phospho-Ser67) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

General Info

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat/Monkey
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-Programmed cell death protein 4-Ser67 (33-82 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	PDCD4
Gene ID:	27250
Uniprot ID:	PDCD4_HUMAN
Immunogen Region:	33-82 aa
Specificity:	Phospho-Pdcd-4 (S67) Polyclonal Antibody detects endogenous levels of Pdcd-4 protein only when phosphorylated at S67.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human PDCD4 around the phosphorylation site of Ser67 at the amino acid range 33-82

Description

Post Translational Modifications	Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens.phosphorylation promotes proteasomal degradation of PDCD4.
Function	Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
Protein Name	Programmed Cell Death Protein 4 Neoplastic Transformation Inhibitor Protein Nuclear Antigen H731-Like Protein 197/15a
Database Links	Reactome: R-HSA-8950505
Cellular Localisation	Nucleus Cytoplasm Shuttles Between The Nucleus And Cytoplasm Predominantly Nuclear Under Normal Growth Conditions And When Phosphorylated At Ser-457
Alternative Antibody Names	Anti-Programmed Cell Death Protein 4 antibody Anti-Neoplastic Transformation Inhibitor Protein antibody Anti-Nuclear Antigen H731-Like antibody Anti-Protein 197/15a antibody Anti-PDCD4 antibody Anti-H731 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance