ATP4B (NM_000705) Human Tagged ORF Clone

CAT#: RC206563

ATP4B (Myc-DDK-tagged)-Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro

AAV Particle: DDK

Datasheet
SDS
Plasmid Map

Product Images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ATP4B
Synonyms ATP6B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206563 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTCTGCAGGAGAAGAAGACGTGTGGCCAGCGCATGGAGGAGTTCCAGCGTTACTGCTGGAACC
CGGACACGGGGCAGATGCTGGGCCGCACCCTGTCCCGGTGGGTGTGGATCAGCCTGTACTACGTGGCCTT
CTACGTGGTGATGACTGGGCTCTTCGCCCTGTGCCTCTATGTGCTGATGCAGACAGTGGACCCGTACACA
CCGGACTACCAAGACCAGCTACGGTCACCAGGGGTAACCTTAAGGCCGGATGTTTACGGGGAGAAAGGCC
TGGAAATTGTCTACAACGTCTCTGATAACAGAACCTGGGCAGACCTCACACAGACTCTCCACGCCTTCCT
AGCAGGCTACTCTCCAGCAGCCCAGGAGGACAGCATCAACTGCACCTCCGAGCAGTACTTCTTCCAGGAG
AGTTTCCGCGCTCCCAACCACACCAAGTTCTCCTGCAAGTTCACGGCAGATATGCTGCAGAACTGCTCAG
GCCTGGCGGATCCCAACTTCGGCTTTGAAGAAGGAAAGCCATGTTTTATTATTAAAATGAACAGGATCGT
CAAGTTCCTCCCCAGCAACGGCTCGGCCCCCAGAGTGGACTGCGCCTTCCTGGACCAGCCCCGCGAGCTC
GGCCAGCCGCTGCAGGTCAAGTACTACCCTCCCAACGGCACCTTCAGTCTGCACTACTTCCCTTATTACG
GGAAGAAAGCCCAGCCCCACTACAGCAACCCCCTGGTGGCAGCGAAGCTCCTCAACATCCCCAGGAACGC
TGAGGTCGCCATCGTGTGCAAGGTCATGGCAGAGCACGTGACCTTCAACAATCCCCACGACCCGTATGAA
GGGAAAGTGGAGTTCAAACTCAAGATTGAGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RC206563 protein sequence
Red=Cloning site Green=Tags(s)

MAALQEKKTCGQRMEEFQRYCWNPDTGQMLGRTLSRWVWISLYYVAFYVVMTGLFALCLYVLMQTVDPYT
PDYQDQLRSPGVTLRPDVYGEKGLEIVYNVSDNRTWADLTQTLHAFLAGYSPAAQEDSINCTSEQYFFQE
SFRAPNHTKFSCKFTADMLQNCSGLADPNFGFEEGKPCFIIKMNRIVKFLPSNGSAPRVDCAFLDQPREL
GQPLQVKYYPPNGTFSLHYFPYYGKKAQPHYSNPLVAAKLLNIPRNAEVAIVCKVMAEHVTFNNPHDPYE
GKVEFKLKIEK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000705
ORF Size 873 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000705.1
RefSeq Size 1509 bp
RefSeq ORF 876 bp
Locus ID 496
UniProt ID P51164
Cytogenetics 13q34
Protein Families Druggable Genome, Transmembrane
Protein Pathways Oxidative phosphorylation
MW 33.4 kDa
Gene Summary The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]

Other Versions

SKU Description Size
RC206563L1 Lenti ORF clone of Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B), Myc-DDK-tagged
    • 10 ug

600,00 EUR

RC206563L2 Lenti ORF clone of Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B), mGFP tagged
    • 10 ug

600,00 EUR

RC206563L3 Lenti ORF clone of Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B), Myc-DDK-tagged
    • 10 ug

600,00 EUR

RC206563L4 Lenti ORF clone of Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B), mGFP tagged
    • 10 ug

600,00 EUR

RG206563 ATP4B (tGFP-tagged) - Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B)
    • 10 ug

500,00 EUR

SC122571 ATP4B (untagged)-Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B)
    • 10 ug

300,00 EUR