BCIP p-toluidine salt, ImmunoTek™

For the colorimetric detection of alkaline phosphatase-labeled molecules in Northern Southern, and Western blotting, in situ hybridization, and Immunohistochemistry. Produces a purple insoluble end product that is detected visually, when used together with Nitroblue tetrazolium.

Synonyms:
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt; X-phosphate p-toluidine salt; X-phos; X-phos PT

CAS Number:
6578-06-9

Molecular Formula:
C15H15BrClN2O4P

Molecular Weight:
433.62

Appearance:
White to off-white powder

Purity:
≥99% (HPLC)

Perchloric Acid Titration (0.1mol/L):
≥99% (Titration)

Solubility (1%, DMF):
Colorless to light yellow, clear, complete

Solubility (1%, dH2O):
Insoluble

Turbidity (1%, DMF):
≤4.0 NTU

Water (KF):
≤1%

Phosphorous (inorganic):
≤ 0.05%

FTIR:
Conform with assigned structure.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C.