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> DNA Damage (8-OHdG) ELISA BioAssay™ Kit

DNA Damage (8-OHdG) ELISA BioAssay™ Kit

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Cat# 474005-96T

Size : 96Tests

Brand : US Biological

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474005 DNA Damage (8-OHdG) ELISA BioAssay™ Kit

Clone Type
Polyclonal
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

The DNA Damage (8-OHdG) ELISA BioAssay™ Kit is a competitive assy for the qualification of 8-OHdG in serum, plasma, urine, culture media, cell lysates, tissue samples and saliva. This BioAssay™ kit offers a quick incubation time, stable reagents, and a user-friendly protocol. Analyze results using an absorbance plate reader.

Detection Range:
0.94-60ng/ml

Sensitivity:
0.59ng/ml

Precision:
Intra-assay CV: <5%
Inter-assay CV: <5%

Test Principle:
This ELISA kit is a competitive assay that can be used for the quantification of 8-OHdG in serum, plasma, urine, culture media, cell lysates, tissue samples and saliva. The ELISA utilizes an 8-hydroxy-2-deoxyguanosine-coated plate and an HRP-conjugated 8-OHdG specific detection antibody. This produces an assay with a useful range of 0.94-60ng/ml, and a sensitivity of 0.59 ng/mL. Other highlights of this kit include a quick incubation time (60 minutes), stable reagents, and a user-friendly protocol.

It is important to note that the 8-OHdG antibody used in this assay recognizes both free 8-OHdG and DNA-incorporated 8-OHdG. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of DNA fragments and free 8-OHdG, concentrations of 8-OHdG reported by ELISA methodology will not coincide with those reported by LC-MS where the single nucleoside is typically measured. This shouldbe kept in mind when analyzing and interpreting experimental results.

Kit Components:
474005A: Microtiter Plate, 1x96wells
*474005B: 8-OHdG Standard (3.06ug/ml), 1x100ul
474005C: 8-OHdG (HRP) Mab Antibody, 1x75ul
474005D: Sample and Standard Diluent, 1x50ml
474005E: 8-OhdG Antibody Diluent, 1x13ml
474005F: Wash Buffer Concentrate, 10X, 1x50ml
474005G: TMB Substrate, 1x13ml
474005H: Stop Solution (1M HCl), 1x13ml

Storage and Stability:
Store *474005B powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Floyd, R.A. Role of oxygen free radicals in carcinogenesis and brain ischemia. FASEB J. 4, 2587-2597 (1990). 2. Spencer, J.P.E., Jenner, A., Chimel, K., et al. DNA strand breakage and base modifi cation induced by hydrogen peroxide treatment of human respiratory tract epithelial cells. FEBS Lett. 374, 233-236 (1995). 3. Epe, B., Ballmaier, D., Roussyn, I., et al. DNA damage by peroxynitrite characterized with DNA repair enzymes. Nucleic Acids Res. 24, 4105-4110 (1996). 4. Beckman, K.B. and Ames, B.N. Oxidative decay of DNA. J. Biol. Chem. 272, 19633-19636 (1997). 5. Shen, J., Deininger, P., Hunt, J.D., et al. 8-hydroxy2’-deoxyguanosine (8-OHdG) as a potential survival biomarker in patients with nonsmall-cell lung cancer. Cancer. 109, 574-580 (2007). 6. Kuo, H.-W., Chou, S.-Y., Hu, T.-W., et al. Urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG) and genetic polymorphisms in breast cancer patients. Mutat. Res. 631, 62-68 (2007). 7. Endo, K., Miyashita, Y., Sasaki, H., et al. Probucol and atorvastatin decrease urinary 8-hydroxy-2’-deoxyguanosine in patients with diabetes and hypercholesterolemia. J. Atheroscler. Thromb. 13, 68-75 (2006). 8. Leinonen, J., Lehtimäki, T., Toyokuni, S., et al. New biomarker evidence of oxidative DNA damage in patients with non-insulin-dependent diabetes mellitus. FEBS Lett. 417, 150-152 (1997). 9. Lee, J., Lee, M., Kim, J.-U., et al. Carvedilol reduces plasma 8-hydroxy-2’-deoxyguanosine in mild to moderate hypertension. A pilot study. Hypertension. 45, 986-990 (2005). 10. Bogdanov, M.B., Beal, M.F., McCabe, D.R., et al. A carbon column-based liquid chromatography electrochemical approach to routine 8-hydroxy-2’-deoxyguanosine measurements in urine and other biologic|matrices: A one-year evaluation of methods. Free Radic. Biol. Med. 27(5/6), 647-666 (1999). 11. Lin, H.-S., Jenner, A.M., Ong, C.N., et al. A highthroughput and sensitive methodology for the quantifi cation of urinary 8-hydroxy-2’-deoxyguanosine:|Measurement with gas chromatography-mass spectrometry after single solid-phase extraction. Biochem. J. 380, 541-548 (2004).

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