Reagents and instruments for immunology, cell biology and molecular biology.

DNA Marker Ladder, 11 Fragments (50-1000bp), Ready to Use

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Cat# D3930-04-5x50ug

Size : 5x50ug

Brand : US Biological

Grade

Molecular Biology Grade

Shipping Temp

Blue Ice

Storage Temp

-20°C

Contains 11 discrete fragments ranging from 50bp to 1000bp with a double intensity reference band at 500bp. Suitable for size determination of digested DNA.

The marker yields the following 11 discrete fragments: 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100, 50.

Quantity of DNA in band:

Fragment (bp) ng/10ul
1000 91
900 66
800 61
700 51
600 43
500 70
400 28
300 23
200 30
100 22
50 15

Recommendations for Use:

1. Vortex gently just prior to use.
2. Do not heat before loading.
3. Apply 10ul of DNA Ladder per well.
4. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5ug/ml ethidium bromide for 30-40 minutes.

*Note: This ladder was standardized using 10ul of DNA per lane on a 0.8cm thick, 13x15cm, 1% agarose gel rrun in TAE buffer.

Storage and Stability:

May be stored at -20°C For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Toxicity and Hazards:

All products should be handled by qualified personnel only, trained in laboratory procedures.

Concentration

~0.05mg/ml

Form

Supplied as a liquid in 10mM EDTA, 0.17% SDS, 0.015% bromophenol blue, 10% glycerol.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.