Size : 50rxns
EasyPure® RNA Kit
Catalog Number： ER101-01
Price：Please inquire first
This kit is designed for the rapid extraction of total RNA from cultured animal cells, tissues and E. coli. The samples are lysed with guanidine isothiocyanate, DNA is digested with DNase I, and RNA is specifically bound to a silica gel membrane spin column. The extracted total RNA is of high purity, free from DNA and protein contamination, and can be used for RT-PCR, qRT-PCR, microarray analysis, Northern blot, and other experiments.
DNase I and DNase I Reaction Buffer are at -18℃or below for one year; others are at room temperature (15℃-30℃) in a dry place for one year.
Dry ice (-70℃)
Component | ER101-01(50 rxns) |
| Binding buffer 4 (BB4) | 40 ml |
| Clean buffer 4 (CB4) | 60 ml |
| Wash buffer 4 (WB4) | 12 ml |
| Proteinase K (20 mg/ml) | 1 ml |
| DNase I (3 units/μl) | 1500 U |
| DNase I Reaction Buffer | 4×1 ml |
| RNase-free Water | 10 ml |
| RNase-free Tube (1.5 ml) | 50 each |
| RNA Spin Columns with Collection Tubes | 50 each |
1 Zhu G, Wang S, Huang Z, et al. Rewiring of the fruit metabolome in tomato breeding[J]. Cell, 2018.(IF 36.21)
2 Zhao K, Wang L, Qiu D, et al. PSW1, an LRR receptor kinase, regulates pod size in peanut[J]. Plant Biotechnology Journal, 2023.(IF 13.80)
3 Wang Y, Wang Y, Pan D, et al. Guide RNA engineering enables efficient CRISPR editing with a miniature Syntrophomonas palmitatica Cas12f1 nuclease[J]. Cell Reports, 2022.(IF 9.99)
4 Zhang Z, Mao L, Qin Y, et al. Comparative transcriptome analysis revealed the role and mechanism of a FeoC-like LuxR-type regulator in intracellular survival of Aeromonas hydrophila[J]. Aquaculture, 2022.(IF 5.13)
5 Wu Z, Zhang Y, Yu H, et al. Programmed genome editing by a miniature CRISPR-Cas12f nuclease[J]. Nature chemical biology, 2021.(IF 15.04)
6 Wang Y, Luo W, Huang L, et al. A novel lncRNA linc-AhRA negatively regulates innate antiviral response in murine microglia upon neurotropic herpesvirus infection[J]. Theranostics, 2021.(IF 11.55)
