Endoglin (CD105, ENG, END) (MaxLight 490)

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Endoglin (CD105) is a homodimeric integral membrane glycoprotein comprised of a 95kD subunit. Endoglin is a component of the TGF-B receptor system that functions as an ancillary receptor influencing binding of the Transforming Growth Factor beta (TGF-B) family of ligands to signaling receptors. Endoglin binds to TGF-B1 and TGF-B3 with high affinity, but does not bind TGF-B2. Endoglin is expressed by vascular endothelial cells and syncytiotrophoblasts of placenta and is weakly expressed by stromalfibroblasts. Endoglin is also expressed by U937 cells and by activated macrophages. Endoglin expression is up-regulated on activated endothelium in tissues undergoing angiogenesis, such as in tumors, or in cases of wound healing or dermal inflammation.

Suitable for use in Flow Cytometry (Not Tested), Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Flow Cytometry (Not Tested): 1:200
Optimal dilutions to be determined by the researcher.

HUVEC cells

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Mab

IgG1,k

44G4

mouse

human

As Reported

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.

Purified by Protein G affinity chromatography.

Whole HOON Pre-B Leukemic cells.

Recognizes human CD105.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.