| Quantity | 100 µl |
|---|---|
| Antibody Type | Polyclonal |
| Host | Guinea pig |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, WB |
| Purification | Stabilized antiserum |
| Reactivity | Human |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Recombinant human keratin K17 |
| Formulation | Contains 0.09% sodium azide and 0.5% BSA |
| UniprotID | Q04695 (Human) |
| Synonym | Keratin, type I cytoskeletal 17, 39.1, Cytokeratin-17, CK-17, Keratin-17, K17, KRT17 |
| Note | Centrifuge prior to opening |
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:100-1:200 |
| Immunohistochemistry (IHC) - frozen | 1:100-1:200 |
| Immunohistochemistry (IHC) - paraffin | 1:50 (microwave treatment recommended) |
| Western Blot (WB) | 1:5,000-1:10,000 |
The antiserum represents an excellent marker to distinguish myoepithelial cells (positive for keratin K17) from luminal epithelium of various glands (mammary, sweat, salivary, bronchial, tracheal, laryngeal, esophageal) and benign from malignant forms of e.g. mammary gland tumors. In the epidermis suprabasal staining is found only in cornifying regions and in the outer root sheath of hair follicles.