High-Density Lipoprotein Cholesterol (HDL-C) BioAssay™ Kit (Double Reagents)

Sensitivity:
The absorbance value △ A is between 0.087~0.153 when testing 1.3mmol/L samples.

Range:
0.65-3.8mmol/L, r2>0.995

Accuracy:
Relative deviation ≤10%.

Precision:
Intra-Assay CV: <3%
Inter-Assay CV: <5%

Kit Components (see Protocol for final kit quantities):
Reagent 1, 24 mLx1 vial:
Good’s buffer, 50mmol/L
Toos, 1mmol/L
MgCl2·6H2O, 15mmol/L
Cholesterol oxidase, ≥3 KU
Peroxidase, ≥5 KU

Reagent 2, 8 mLx1 vial:
Good’s buffer, 50mmol/L
4-amino-antipyrine, 0.2mmol/L
MgCl2·6H2O, 15mmol/L
Cholesterol esterase, ≥3 KU
Surfactant, 0.1%

Standard, 1 mLx1 vial:
Cholesterol, 1.05mmol/L

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.