JC-1 Mitochondrial Membrane Potential Detection Kit (100 assays)
Cat# 30001
Size : 1MG
Brand : Biotium
JC-1 Mitochondrial Membrane Potential Detection Kit
JC-1 dye is used for fluorescence-based measurement of mitochondrial membrane potential changes in cells, a key measure of cell viability.
Product Attributes
| Apoptosis/viability marker | Mitochondrial potential |
|---|---|
| For live or fixed cells | For live/intact cells |
| Detection method/readout | Microplate reader (fluorescence), Fluorescence microscopy, Flow cytometry |
| Assay type/options | Endpoint assay |
| Potential dependence | Mitochondrial potential-dependent |
| Indicator type | Ratiometric |
| Colors | Green/Red |
| Excitation/Emission | 510/527 nm (monomer); 585/590 nm (aggregate) |
| Storage Conditions | Store at 2 to 8 °C, Protect dye component from light |
Product Description
JC-1 Mitochondrial Membrane Potential Detection Kit is used to measure mitochondrial membrane potential changes in cells, a key indicator of cell viability.
Features
- Rapid, simple measurement of mitochondrial membrane potential/cell viability
- Healthy cells stain red & green, dead cells stain green
- For fluorescence microscopy, flow cytometry, or fluorescence microplate reader
Spectral properties
- Ex/Em (cytoplasm): 510/527 nm (green)
- Ex/Em (polarized mitochondria): 585/590 nm (red)
Kit components
- JC-1, 100X in DMSO
- 10X Assay Buffer
The loss of mitochondrial membrane potential is a hallmark for apoptosis. It is an early event preceding phosphatidylserine externalization and coinciding with caspase activation. In non-apoptotic cells, JC-1 accumulates as aggregates in the mitochondrial membranes, resulting in red fluorescence. The brightness of red fluorescence is proportional to the potential and varies among different cell types. However, in apoptotic and necrotic cells, which have diminished mitochondrial membrane potential, JC-1 exists in the green fluorescent monomeric form. This kit provides a step-by-step protocol and ready-to-use reagents for performing assays for flow cytometry, fluorescence microscopy, or fluorescence microplate reader.
Biotium also offers Aquaphile™ JC-1, an improved and more soluble formulation of the JC-1 dye which minimizes false positive J-aggregate signal outside of cells. Biotium also sells JC-1 chloride salt and JC-1 iodide salt.
References
Download a list of curated JC-1 Mitochondrial Membrane Potential Kit References.
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