Reagents and instruments for immunology, cell biology and molecular biology.

Milenia® GenLine HybriDetect 2T

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Cat# MGHD21-5

Size : 5x100tests

Brand : Milenia Biotec

( excludes VAT and shipping costs)

100

The HybriDetect 2T is a simple and quick tool to develop your own rapid test. The 2T is designed for simultaneous detection of two analytes. Various molecules can be detected: Proteins, Antibodies, Genetic amplification products

Safety Data Sheet

Overview

Name of Product

HybriDetect 2T

Catalog Number

MGHD2 1

Short Info

Method/Platform

Lateral flow, sandwich or competetive

Range/Assay Sensivity

5 pg DNA, equivalent to agarose gel electrophoresis

Test Principle

HybriDetect 2T is a ready-to-use, universal test strip (dipstick) based on lateral flow technology using gold particles. The dipstick can be used to develop qualitative or quantitative rapid test systems for simultaneous detection of two different analytes such as proteins, antibodies or gene amplification products. The results can be interpreted qualitative or quantitative.

Documents

Protocol

Brief Instructions

Click here for the Instructions for Use

Storage

2-8°C

Components

HybriDetect 2T dipsticks, HybriDetect 2T assay buffer

References

A novel isothermal assay of Borrelia burgdorferi by recombinase polymerase amplification with lateral flow detection. International Journal of Molecular Sciences, 17(8). (2016)
Liu, W., Liu, H. X., Zhang, L., Hou, X. X., Wan, K. L., & Hao, Q.
A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchus xylophilus by loop-mediated isothermal amplification. Phytopathology, 99(12), 1365–1369. (2009)
Kikuchi, T., Aikawa, T., Oeda, Y., Karim, N., & Kanzaki, N.
A Rapid Molecular Assay for Detecting the Mediterranean Fanworm Sabella spallanzanii Trialed by Non-Scientist Users (2022)
Zaiko, A.
An integrated isothermal nucleic acid amplification test to detect HPV16 and HPV18 DNA in resource-limited settings (2023)
Kundrod, K. A., Barra, M., Wilkinson, A., Smith, C. A., Natoli, M. E., Chang, M. M., Coole, J. B., Santhanaraj, A., Lorenzoni, C., Mavume, C., Atif, H., Montealegre, J. R., Scheurer, M. E., Castle, P. E., Schmeler, K. M., & Richards-Kortum, R. R.
Comparison of Three Terminal Detection Methods Based on Loop Mediated Isothermal Amplification (LAMP) Assay for Spring Viremia of Carp Virus (SVCV). (2019). Turkish Journal of Fisheries and Aquatic Sciences, 19(9), 805–816. (2019)
Lu Liu1, Yuyan Xu2, Weifang Zhong1, Lina Li1, Weizhe Li1, Qin Xiao1
Development of an isothermoal amplification-based assay for rapid visual detection of an Orf virus. Virology Journal, 13(1), 1–6. (2016)
Yang, Y., Qin, X., Wang, G., Jin, J., Shang, Y., & Zhang, Z.
Development of Duplex LAMP Technique for Detection of Porcine Epidemic Diarrhea Virus (PEDV) and Porcine Circovirus Type 2 (PCV 2) (2022)
Areekit, S., Tangjitrungrot, P., Khuchareontaworn, S., Rattanathanawan, K., Jaratsing, P., Yasawong, M., Chansiri, G., Viseshakul, N., & Chansiri, K.
Development of Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick Assay for a Rapid and Sensitive Detection of Cystic Echinococcosis in Livestock in Kenya (2022)
Badoul, N. A., Kagira, J., Ng’Ong’A, F., & Dinka, H.
Development of real-time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus. Virology Journal, 14(1), 1–10. (2017)
Yang, Y., Qin, X., Song, Y., Zhang, W., Hu, G., Dou, Y., … Zhang, Z.
Improved nucleic acid testing strategies to detect and discriminate veterinary relevant Mycobacterium tuberculosis complex members (2014)
Costa P. M. N.
Isothermal Amplification and Lateral Flow Nucleic Acid Test for the Detection of Shiga Toxin-Producing Bacteria for Food Monitoring (2022)
Denti, M. A., Park, H. G., Petrucci, S., Dikici, E., Daunert, S., Deo, S. K., & Macdonald, J. T.
Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection of Cryptosporidium Species in Kenyan Children Presenting with Diarrhea. Journal of Tropical Medicine, 2018. (2018)
Mamba, T. S., Mbae, C. K., Kinyua, J., Mulinge, E., Mburugu, G. N., & Njiru, Z. K.
Multiplex PCR-Lateral Flow Dipstick Method for Detection of Thermostable Direct Hemolysin (TDH) Producing V. chaemolyticus (2023)
Saetang, J., Sukkapat, P., Palamae, S., Singh, P., Senathipathi, D. N., Buatong, J., & Benjakul, S.
Nucleic Acid Lateral Flow Immunoassays: Nachweisentwicklung, Limitationen und methodische Neuerungen (2021)
Breitbach, A.
Point-of-Care to Amplify and Detect High-Risk HPV DNA and mRNA (2020)
Kundrod K.A.
Rapid and sensitive detection of Candidatus Liberibacter asiaticus by loop mediated isothermal amplification combined with a lateral flow dipstick. BMC Microbiology, 14(1), 1–9. (2014)
Rigano, L. A., Malamud, F., Orce, I. G., Filippone, M. P., Marano, M. R., Do Amaral, A. M., … Vojnov, A. A.
Rapid and sensitive detection of human African trypanosomiasis by loop-mediated isothermal amplification combined with a lateral-flow dipstick (2011)
Njiru Z. K.
Rapid and sensitive detection of infectious spleen and kidney necrosis virus by loop-mediated isothermal amplification combined with a lateral flow dipstick. Archives of Virology, 155(3), 385–389. (2010)
Ding, W. C., Chen, J., Shi, Y. H., Lu, X. J., & Li, M. Y.
Rapid and sensitive detection of Vibrio vulnificus by loop-mediated isothermal amplification combined with lateral flow dipstick targeted to rpoS gene. Molecular and Cellular Probes, 25(4), 158–163. (2011)
Surasilp, T., Longyant, S., Rukpratanporn, S., Sridulyakul, P., Sithigorngul, P., & Chaivisuthangkura, P.
Rapid detection and differentiation of carp oedema virus and cyprinid herpes virus-3 in koi and common carp. Journal of Fish Diseases, 41(5), 761–772. (2018)
Soliman, H., & El-Matbouli, M.
Rapid detection of cagA-positive Helicobacter pylori based on duplex recombinase aided amplification combined with lateral flow dipstick assay (2022)
Zhu, X., Zhao, Y., Zhu, C., Wang, Y., Liu, Y., & Su, J.
Rapid detection of fluoroquinolone resistance in Mycobacterium tuberculosis using a novel multienzyme isothermal rapid assay (2023)
Li, M. chao, Lu, Y., Liu, H. can, Lin, S. qiang, Qian, C., Nan, X. tian, Li, G. lian, Zhao, X. qin, Wan, K. L., & Zhao, L. li.
Rapid detection of methicillin-resistant Staphylococcus aureus in positive blood-cultures by recombinase polymerase amplification combined with lateral flow strip (2022)
Srisrattakarn, A., Panpru, P., Tippayawat, P., Chanawong, A., Tavichakorntrakool, R., Daduang, J., Wonglakorn, L., & Id, A. L.
Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis. Malaria Journal, 13(1), 1–9. (2014)
Kersting, S., Rausch, V., Bier, F. F., & Von Nickisch-Rosenegk, M.
Rapid Diagnostic Test for Hepatitis B Virus Viral Load Based on Recombinase Polymerase Amplification Combined with a Lateral Flow Read-Out (2022)
Mayran, C., Foulongne, V., de Perre, P. van, Fournier-Wirth, C., Molès, J. P., & Cantaloube, J. F.
Rapid Visual Detection of Streptococcus suis and Actinobacillus pleuropneumoniae Through Duplex Recombinase Polymerase Amplification Combined with Lateral Flow Dipsticks (2022)
Zhang, S., Xie, H., Liu, M., Zheng, A., Yan, H., Duan, M., Wei, X., Teng, Z., Zhang, H., & Xia, X.
Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids. Parasites and Vectors, 11(1), 1–8. (2018)
Soliman, H., Kumar, G., & El-Matbouli, M.
Shrimp Taura syndrome virus detection by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick. Journal of Virological Methods, 153(2), 214–217. (2008)
Kiatpathomchai, W., Jaroenram, W., Arunrut, N., Jitrapakdee, S., & Flegel, T. W.

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