| Quantity | 50 µg |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, WB |
| Purification | Affinity chromatography |
| Reactivity | Human |
| No reactivity | Mouse |
| Storage before reconstitution | 2-8°C until indicated expiry date |
| Storage after reconstitution | Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | HEA125 |
| Immunogen | HT-29 colon carcinoma cell line |
| Concentration | 50 µg/ml after reconstitution with 1 ml dist. water |
| Formulation | Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| Synonym | Human Epithelium Antigen, HEA, 17-1A, EPG34, CD326, TACSTD1 |
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:10-1:50 (1-5 µg/ml) |
| Immunohistochemistry (IHC) - paraffin | 1:10-1:50 (1-5 µg/ml; protease treatment recommended) |
| Western Blot (WB) | 1:200-1:3.000 (0.02-0.25 µg/ml) |
The Ep-CAM (HEA125) antibody recognizes the epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen, EPG34, CD326, and TACSTD1). This antigen is widely expressed on cells of epithelial origin and tumors derived therefrom. HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative.
The antibody can be used as alternative to the anti-EpCAM antibody clone BerEp4.
Polypeptide reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM).
Reactivity on cultured cell lines: all carcinoma cell lines tested so far; particularly strong reaction with colon carcinoma cell lines.