Phaseolus limensis (LBA)

Phaseolus limensis agglutinin (LBA) is isolated from lima bean seeds and purified by affinity chromatography. LBA is composed of 30,000 Da subunits, of which two are joined by a disulfide bond. Each subunit contains a cysteine residue which is required for both it’s carbohydrate- and metal-ion-binding activities. It has a carbohydrate specificity of terminal GalNAcalpha(1,3)Gal that elutes with N-acetylgalactosamine. LBA agglutinates blood group A erythrocytes.

Source:
Lima bean

Blood Specificity:
A1 > A2 >> B

Sugar Specificity:
GalNAc alpha(1,3) [Fuc alpha(1,2)] Gal

Inhibitory Carbohydrate:
N-Acetylgalactosamine

Divalent Ions:
Ca++ Mn++

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.