Trypsin Activity Assay Kit
Cat# E-BC-K843-M-48T
Size : 48T
Brand : Elabscience
| Detection Principle | Trypsin catalyzes the substrate to form p-nitroaniline, which has a certain light absorption value at 400-410 nm wavelength. Since the absorbance of p-NA is proportional to the content, the activity of trypsin can be calculated by measuring the p-NA produced per unit time. |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (400-410 nm, optimum wavelength: 405 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.86 U/L |
| Detection Range | 1.86-100.65 U/L |
| Precision | inter-assay CV: 7.2-11.2% | intra-assay CV: 3.9-5.1% |
| Sample Volume | 20 μL(cell homogenate) |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse small intestine tissue homogenate | 2-5 |
| 10% Mouse large intestine tissue homogenate | 2-5 |
| 10% Rat intestine tissue homogenate | 2-5 |
| 10% Rat chyme tissue homogenate | 2-5 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
{{antibodyDetailsPage.numTotal}} Results
- {{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® 430
Elab Fluor® 488
Elab Fluor® 568
Elab Fluor® 594
Elab Fluor® 647
Elab Fluor® 680
Elab Fluor® 700
Elab Fluor® 750
Elab Fluor® Red 780
Elab Fluor® Violet 450
Elab Fluor®488
Elab Fluor®594
Elab Fluor®647
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
- {{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
