TXK (Protein Tyrosine Kinase 4) BioAssay™ ELISA Kit (Human)

The TXK (Protein Tyrosine Kinase 4) BioAssay™ ELISA Kit (Human) is a quantitative sandwich assay for the detection of TXK in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples.

Sensitivity:
0.188ng/ml

Detection Range:
0.313-20ng/ml

Specificity:
This assay has high sensitivity and excellent specificity for detection of human TXK. No significant cross-reactivity or interference between TXK and analogues was observed.

Precision:
Intra-Assay: CV<8%
Inter-Assay: CV<10%

Test Principle:
This ELISA kit employs the sandwich enzyme-linked immune-sorbent assay technology. Anti-TXK antibody was pre-coated onto 96-well plates. The biotin conjugated anti-TXK antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to
produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TXK amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TXK can be calculated.

Kit Components:
Micro ELISA Plate(Dismountable), 8×12
Lyophilized Standard, 2x vial
Sample / Standard dilution buffer, 1x20ml
Biotin-labeled antibody (Concentrated), 1x120ul
Antibody dilution buffer, 1x10ml
HRP-Streptavidin Conjugate(SABC), 1x120ul
SABC dilution buffer, 1x10ml
TMB substrate, 1x10ml
Stop solution, 1x10ml
Wash buffer (25X), 1x30ml
Plate Sealer, 1x5pieces

Storage and Stability:
Store unopened kit at 4ºC; store at -20ºC once opened. Store unopened standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.