Wheat Germ Agglutinin, CF®770 Conjugate

Cat# 29059

Size : 5x1MG

Brand : Biotium


Wheat Germ Agglutinin (WGA) Conjugates

A wheat germ agglutinin (WGA) lectin labeled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine and stains yeast bud scars as well as the cell membrane of gram+ bacteria and mammalian cells.

Product Attributes

Probe cellular localization

Membrane/cell surface

For live or fixed cells

For fixed cells, For live/intact cells

Cell permeability

Membrane impermeant

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Colors

Blue, Green, Orange, Red, Far-red, Near-infrared

Product Description

Wheat germ agglutinin (WGA) is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram bacteria and mammalian cells.

  • Stain yeast bud scars as well as the cell membrane of gram bacteria and mammalian cells or tissues
  • Suitable for Western Blot, ELISA, immunohistochemistry and other standard immunoassay applications
  • A retrograde or anterograde neuronal tracer
  • Withstands fixation and permeabilization
  • Choice of 13 CF® dyes from UV to near-infrared or HRP
  • Superior CF® dyes are bright, photostable, and water-soluble

WGA is commonly used to label glycoproteins for imaging of the plasma membrane in live or fixed cells, for staining of tissue sections, or for western blotting.  WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae.

Find the Right Stain for Your Application

WGA and other lectins are carbohydrate binding proteins that recognize specific sugar moieties on glycoproteins.  The presence and distribution of these targets vary between cell types and tissues. As a result, other cell surface stains or other lectin conjugates, Concanavalin A (Con A) Conjugates and PNA Lectin Conjugates, may produce better surface staining and may be more appropriate for your cell type. Lectin conjugates can be used to selectively stain the cell surface of live cells, and withstand fixation and permeabilization. When cells are fixed and permeabilized before staining, fluorescent lectins stain both cell surface and organelles in the secretory pathway. Lectins may be toxic or stimulatory to live cells depending on cell type. To find the right stain for your application, see our Membrane & Cell Surface Stains Comparison, or download our Membrane & Surface Stains Brochure. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.

Wheat Germ Agglutinin Conjugates

Product Conjugation Ex/Em Size Catalog No. Purchase
HRP WGA HRP N/A 1 mg 29073 Purchase 29073
CF®350 WGA CF®350 347/448 nm 1 mg 29021-1 Purchase 29021-1
5 mg 29021 Purchase 29021
CF®405S WGA CF®405S 404/431 nm 1 mg 29027-1 Purchase 29027-1
5 mg 29027 Purchase 29027
CF®405M WGA CF®405M 408/452 nm 1 mg 29028-1 Purchase 29028-1
5 mg 29028 Purchase 29028
CF®488A WGA CF®488A 490/515 nm 1 mg 29022-1 Purchase 29022-1
5 mg 29022 Purchase 29022
CF®532 WGA CF®532 527/558 nm 1 mg 29064-1 Purchase 29064-1
5 mg 29064 Purchase 29064
CF®555 WGA CF®555 555/565 nm 5 mg 29076 Purchase 29076
1 mg 29076-1 Purchase 29076-1
CF®568 WGA CF®568 562/583 nm 5 mg 29077 Purchase 29077
1 mg 29077-1 Purchase 29077-1
CF®594 WGA CF®594 593/614 nm 1 mg 29023-1 Purchase 29023-1
5 mg 29023 Purchase 29023
CF®633 WGA CF®633 630/650 nm 1 mg 29024-1 Purchase 29024-1
5 mg 29024 Purchase 29024
CF®640R WGA CF®640R 642/662 nm 1 mg 29026-1 Purchase 29026-1
5 mg 29026 Purchase 29026
CF®680 WGA CF®680 681/698 nm 1 mg 29029-1 Purchase 29029-1
5 mg 29029 Purchase 29029
CF®680R WGA CF®680R 680/701 nm 1 mg 29025-1 Purchase 29025-1
5 mg 29025 Purchase 29025
CF®770 WGA CF®770 770/797 nm 1 mg 29059-1 Purchase 29059-1
5 mg 29059 Purchase 29059

 

CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.

References

CF® Dye WGA in bacteria
1. BMC Microbiol. (2014), DOI: 10.1186/1471-2180-14-56

CF® Dye WGA in fungi
2. Eur J Plant Pathol. (2017), DOI: 10.1007/s10658-017-1226-5
3. Sci Rep. (2017), DOI: 10.1038/s41598-017-16401-6.

CF® Dye WGA in cells & tissues
4. J Tissue Eng. (2012), DOI: 10.1177/2041731412455354
5. Nanotechnology (2013), DOI: 10.1088/0957-4484/24/28/285101
6. BMC Biol. (2016), DOI: 10.1186/s12915-016-0332-8
7. Macromol. Biosci. (2016), DOI: 10.1002/mabi.201600333
8. PLoS One (2016), DOI: 10.1371/journal.pone.0156456
9. Sci Rep. (2017), DOI: 10.1038/srep42941
10. MBio. (2017), DOI: 10.1128/mBio.02030-16
11. FEBS J. (2019) DOI: 10.1111/febs.15088
12. Cell Physiol Biochem.(2019) DOI: 10.33594/000000070

 

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