Phaseolus vulgaris (PHA-L)

Cat# 518660-10mg

Size : 10mg

Brand : US Biological



Grade
Affinity purified

Shipping Temp
Blue Ice

Storage Temp
-20°C

PHA-L
Phaseolus vulgaris lectin (PHA-L) is affinity purified lectin made up of four subunits with carbohydrate specificity for complex oligosaccharides. PHA-L is known as leucoagglutinin and has high mitogenic and leucoagglutinating activity, and low erythroagglutinating activity. This lectin exhibits no agglutination with human erythrocytes. PHA-E differs from PHA-L in that the leucoagglutinin reacts strongly with Tamm-Horsfall glycoprotein and reacts weakly with porcine thyroglobulin. PHA-L binds to fetuin but does not precipitate the glycoprotein. This lectin is strongly inhibited by trisaccharide Gal β(1,4)GlcNAcβ(1,2)Man, and although this structure is present in many glycoproteins, PHA-L does not react with all of them. This lectin reacts strongly with certain carcinoma cell lines of high metastatic potential and has been proposed as a probable therapeutic agent. It has shown potential to inhibit graft vs. host reaction in transplantation studies and has also shown to promote the production of cytotoxic agents which could be useful in cancer therapy.

Carbohydrate Specificity:
Complex N-Glycans

Inhibitory Carbohydrate:
Gal β(1,4)GlcNAcβ(1,2)Man

Divalent Ions:
Ca2+, Mn2+

Activity:
<0.5ug/ml will agglutinate neuraminidase-treated human erythrocytes

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source
Kidney bean

Purity
Affinity purified (≥95)

Form
Supplied as a lyophilized powder


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.