Polygonatum cyrtonema Lectin (PCL) - Pure

Polygonatum cyrtonema lectin (PCL) is isolated from Solomon's Seal seeds and affinity purified. This lectin is a dimer with an estimated molecular weight of 4.2 kDa and has agglutinated rabbit erythrocytes. PCL is specific to mannose/sialic acid and is one of the GNA-related lectins (formerly named the mannose binding lectin family). In the β-prism II fold of PCL exists three carbohydrate binding sites, with two sites holding the essential amino acids for recognizing mannose. The third carbohydrate binding site contains amino acid substitutions that causes PCL to have less affinity for mannose than other GNA-related lectins, but also allows for sialic acid binding. This non-mannose binding site sets PCL apart, giving it potential as an influenza A treatment that utilizes competitive binding of sialic-acid specific retrovirus infections. In cancer research, PCL has been shown to induce apoptosis via activation of the caspase-dependent, Ras-Raf, PI3K-Akt, and mitochondria-mediated p53 pathways. PCL also induces autophagic effects by blocking the same Ras-Raf and PI3K-Akt pathways that induce apoptosis, so intricate maintenance of these pathways is essential for PCL-induced cancers to progress. Pure PCL can be used for blotting and immunohistochemistry techniques. This product comes in a lyophilized form and is stable for more than three years when stored below -20°C.

Specifications:

• Source: Polygonatum cyrtonema (Soloman's Seal)
• Carbohydrate Specificity: Mannose, Sialic acid
• Inhibitory Carbohydrate: Mannose, Sialic acid, Thyroglobulin
• Divalent ions required: None

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 °C for short term use. Clarify by centrifugation, if needed.

Application

Glycobiology, Immunohistochemistry, Immunocytochemistry, Blotting

References

Chen, Y., Lu, K., Li, J., Liang, D., luo, H., Wang, X., Wang, X., & Bao, J. (2017). Structure and function analysis of Polygonatum cyrtonema lectin by site-directed mutagenesis. Acta Biochimica Et Biophysica Sinica, 49(12), “1111. https://doi.org/10.1093/abbs/gmx116

Ding, J., Bao, J., Zhu, D., Zhang, Y., & Wang, D. C. (2010). Crystal structures of a novel anti-HIV mannose-binding lectin from Polygonatum cyrtonema Hua with unique ligand-binding property and super-structure. Journal of structural biology, 171(3), “317. https://doi.org/10.1016/j.jsb.2010.05.009

Liu, T., Wu, L., Wang, D., Wang, H., Chen, J., Yang, C., Bao, J., & Wu, C. (2016). Role of reactive oxygen species-mediated MAPK and NF-κB activation inpolygonatum cyrtonemalectin-induced apoptosis and autophagy in human lung adenocarcinoma A549 cells. Journal of Biochemistry, 160(6), “324. https://doi.org/10.1093/jb/mvw040

Xu, H. L., Li, C. Y., He, X. M., Niu, K. Q., Peng, H., Li, W. W., Zhou, C. C., & Bao, J. K. (2012). Molecular modeling, docking and dynamics simulations of GNA-related lectins for potential prevention of influenza virus (H1N1). Journal of molecular modeling, 18(1), “37. https://doi.org/10.1007/s00894-011-1022-7

Wang, S. Y., Yu, Q. J., Bao, J. K., & Liu, B. (2011). Polygonatum cyrtonema lectin, a potential antineoplastic drug targeting programmed cell death pathways. Biochemical and biophysical research communications, 406(4), “500. https://doi.org/10.1016/j.bbrc.2011.02.049

Zhang, H., Du, X., Sun, T.-T., Wang, C.-L., Li, Y., & Wu, S.-Z. (2017). Lectin PCL inhibits the Warburg effect of PC3 cells by combining with EGFR and inhibiting HK2. Oncology Reports, 37(3), “1771. https://doi.org/10.3892/or.2017.5367