Sanger Sequencing cleanup

Sanger Sequencing cleanup

 Kits and reagents for Dye Terminator Removal 

 

Performa® DTR – The gold standard in gel filtration based dye terminator removal!
 
Performa® DTR utilizes a fully hydrated gel filtration matrix that provides a convenient and simple method of dye terminator removal for CE sequencing clean-up. Performa DTR ensures removal of BigDye® v3.1 or other dye terminators, along with dNTPs, salts and other low molecular weight materials from sequencing reactions. DNA primers and fragments up to 20 bases, buffers, nucleotides labeled with biotin, isotopes and other assorted markers are also eliminated. To minimize the potential for interference with sequencing applications, no preservatives, salts or buffers are used in the preparation of Performa DTR.
 
Features
  • Long read lengths – consistently achieve contiguous read lengths >900 bases in >95%
  • More readable sequence – identify bases close to the primer with excellent signal strength
  • Quick 2-spin protocol – start to finish in only 5 minutes
  • Minimal DNA manipulation – just add your sample and spin
  • Ready-to-use – pre-packed with a fully hydrated gel filtration matrix
  • Flexible formats – single, 96- and 384-well formats
  • Convenient – spin directly into the ABI Prism® 96-well Optical Reaction Plate or comparable plates and load directly onto capillary sequencers
  • Effective dye terminator removal of various chemistries – BigDye® v3.1, BigDye v1.1, GenomeLab, DTCS Quick Start Kit and Methods Development Kit
performa-dye-terminator-sanger

Downstream Applications
  • DNA Sequencing
  • SNP Genotypng




Performa DTR Multi-Well Systems

Overview

The plates are aivalable in three formats :
 
Performa DTR Ultra 96-Well Plates
For a total reaction volume of 5-20 µl; for special sequencing protocols requiring high Dye Terminator volumes (also available as kit including lids and receiver plates, which can be purchased separately as well)
Performa DTR V3 96-Well Short Plates
For a total reaction volume of 5-15 µl (also available as kit including lids and receiver plates, which can be purchased separately as well)
Performa DTR 384-Well Plates
For a total reaction volume of 5-10 µl (only available without receiver plate, which can be purchased separately)

Shortest Time to Completion
  • Ready-to-Use: Pre-packed with a fully hydrated gel filtration matrix
  • Simplest protocol with minimal handling
  • Only 3 steps!
 
Format Size Product code
Performa® DTR ultra
96-Well plate 2 plates 54789
10 plates 55373
10 plates (no receiver) 74521
50 plates (no receiver) 86077
384-well plate 2 plates (no receiver) 52571
Performa® DTR V3
96-Well short plate 2 plates 63887
10 plates 89939
10 plates (no receiver) 47938 Plates Flowchart
Collect purified products directly into ABI Prism MicroAmp® Optical Reaction Plate or comparable plates, load directly onto your capillary sequencing platform.
50 plates (no receiver) 80808

Performa® DTR Individual Cartridge Systems

Description
Performa DTR gel filtration cartridges are 800 µL spin columns assembled in 1.5 mL microcentrifuge tubes. These columns provide optimal performance for removal of unincorporated BigDye® v1.1, v3.0, and v3.1 and other dye terminators, dNTPs, salts, and other low molecular weight materials from sequencing reactions. These columns also remove DNA primers and fragments up to 20 bases, buffers, and nucleotides labeled with biotin, isotopes, and other assorted markers.

The spin columns are pre-packed with a fully hydrated matrix to afford optimal handling and performance characteristics. To minimize the potential for interference with sequencing applications, no preservatives, salts, or buffers are used in the preparation of these columns.


 
Size Product code
Spin columns
36/pk 73328
108/pk 13266
Gel filtration Cartbridges
36/pk 98780
108/pk 42453 Column Flowchart
At the end of the procedure the sample is ready for immediate loading onto the capillary sequencing platform