Visualize apoptosis in real-time with NucView
The ONLY caspase substrates for real-time apoptosis detection
NucView Caspase-3 Substrates offer a validated solution for real-time monitoring of apoptosis in live cells by microscopy or flow cytometry.
- Simple: No wash, homogeneous assay for real-time or end-point apoptosis detection in live cells
- Fast and Fixable: Formaldehyde fixable staining in only 15 minutes
- Non-toxic: Useful for long multi-day experiments
- 3 color options: blue, green, or red fluorescence
Available in 10 µL format, test it !
How it works ?
When introduced to cells, the NucView substrate is initially non-fluorescent and is able to penetrate the plasma membrane and enter the cytoplasm. During apoptosis, caspase-3/7 cleaves the substrate and releases the high-affinity DNA dye which leads to nuclear fluorescent staining.
NucView® substrates consist of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with fluorescence. Thus, NucView caspase-3 substrates are bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis.
In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView® caspase-3 substrates can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. Staining is compatible with subsequent fixation and permeabilization for immunostaining.
NucView® substrates are offered as solutions in DMSO or phosphate-buffered saline (PBS). The substrates in PBS are formulated for use in cells that are sensitive to DMSO toxicity. In non-DMSO sensitive cell types, adding DMSO during the substrate incubation may enhance NucView® staining.