25% off on more than 2000 secondary antibodies

25% off on more than 2000 secondary antibodies

Offer n°20170728-Rockland-Secondary valid until 30/09/2017

Choosing a secondary antibody is straightforward:   select a secondary antibody that recognizes the host species used to produce the primary antibody of interest, i.e. choose an anti-mouse secondary antibody to detect a mouse monoclonal primary antibody.
However, identifying the optimal secondary antibody requires knowledge of the detection assay. For example:   Western blot and ELISA can be performed using colorimetric, chemiluminescent, and fluorescence reporter systems, while immunofluorescence and flow cytometry are generally limited to fluorescent reporter labels. In all of these instances, a conjugated secondary antibody is required.

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Dylight  Secondary
Antibodies
HRP Secondary Antibodies ATTO Dye Secondary Antibodies

How to Select the Optimal  Secondary Antibody

The following variables should be considered in the approximate order of importance:

Criteria Note
Host Secondary antibody should specifically detect the species that produced the primary antibody
Polyclonal or monoclonal Polyclonal antibodies are sufficient for most needs
Monoclonal antibodies are more difficult to produce but are highly specific and yield more consistent results over time
Detection systems and Protocols Does detection system use color (i.e. by dye), light (i.e. ELISA) or fluorescence (i.e. immunofluorescence) ?
Other specificities Subclass, Fab or F(ab')2 fragments, reduced cross reaction to other species