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Coronavirus IBV nsp13 Gene Tagged ORF Clone

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Cat# VC102263

Size : 10ug

Coronavirus IBV nsp13 Gene Tagged ORF Clone

CAT#: VC102263

TrueORF^®

Myc-DDK-tagged ORF clone for coronavirus nsp13 [Infectious bronchitis virus], codon optimized for human cell expression, NP_740633

Reconstitution Protocol

Product Images

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Symbol	coronavirus nsp13
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC102263 represents NCBI reference of NP_740633 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGCATGGACTTGTGGCTACAATATGCCAGAGCTGTACAAGGTTCAGAACTGCGTTATGGAACCCT GCAATATCCCTAACTACGGCGTGGGAATCACCCTGCCCAGCGGGATTCTGATGAACGTGGCCAAGTATAC TCAGCTGTGCCAATACTTGAGCAAGACTACCATATGCGTGCCACACAATATGAGGGTGATGCATTTCGGG GCTGGAAGCGATAAGGGAGTTGCTCCCGGATCAACCGTTCTCAAGCAGTGGCTGCCAGAGGGTACACTGC TCGTAGACAACGATATAGTCGATTATGTCAGTGATGCGCACGTTTCCGTACTGTCCGACTGCAATAAGTA CAACACCGAGCACAAATTCGATCTTGTGATCTCTGATATGTACACAGATAACGACTCAAAGAGAAAGCAC GAAGGCGTGATTGCCAACAACGGCAATGACGATGTGTTTATCTATCTCAGTTCATTTCTCAGGAACAACC TGGCTCTGGGGGGCAGTTTCGCCGTTAAAGTGACCGAAACCAGCTGGCACGAGGTGCTGTATGACATCGC CCAAGACTGTGCGTGGTGGACAATGTTCTGCACTGCTGTTAACGCAAGTTCCTCAGAGGCTTTCTTGATC GGGGTTAATTATTTGGGCGCAAGTGAGAAAGTCAAGGTTAGTGGCAAAACCCTTCATGCAAATTACATCT TCTGGAGAAACTGCAACTATCTGCAGACATCAGCCTATAGTATCTTCGACGTAGCTAAGTTCGATCTCCG GCTTAAGGCAACCCCTGTCGTCAATCTGAAGACTGAACAGAAGACGGACCTGGTTTTCAACCTGATCAAG TGCGGAAAACTGCTCGTCAGAGACGTCGGCAACACCTCTTTCACAAGTGACTCATTTGTGTGTACGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC102263 representing NP_740633 Red=Cloning sites Green=Tags MSAWTCGYNMPELYKVQNCVMEPCNIPNYGVGITLPSGILMNVAKYTQLCQYLSKTTICVPHNMRVMHFG AGSDKGVAPGSTVLKQWLPEGTLLVDNDIVDYVSDAHVSVLSDCNKYNTEHKFDLVISDMYTDNDSKRKH EGVIANNGNDDVFIYLSSFLRNNLALGGSFAVKVTETSWHEVLYDIAQDCAWWTMFCTAVNASSSEAFLI GVNYLGASEKVKVSGKTLHANYIFWRNCNYLQTSAYSIFDVAKFDLRLKATPVVNLKTEQKTDLVFNLIK CGKLLVRDVGNTSFTSDSFVCTM TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	NC_001451
ORF Size	909 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@biotrend-usa.com The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NC_001451.1, NP_740633
RefSeq ORF	909 bp
MW	33.8 kDa

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System TrueClones: Full length cDNA clones (Human/Mouse/Rat), expressing native proteins pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
SDS for VC102263

Resources

cDNA Clone Resources
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones Protocol: Lentivirus packaging Product Sheet: AAV AAV Biosafety

Other Versions

SKU	Description	Size
VC102242	Myc-DDK-tagged ORF clone of viral ORF for spike protein [Infectious bronchitis virus], codon optimized for human cell expression, NP_040831	10 ug	1 113,00 EUR
VC102243	Myc-DDK-tagged ORF clone of viral ORF for 3a protein [Infectious bronchitis virus], codon optimized for human cell expression, NP_040832	10 ug	165,00 EUR
VC102244	Myc-DDK-tagged ORF clone of viral ORF for 3b protein [Infectious bronchitis virus], codon optimized for human cell expression, NP_040833	10 ug	165,00 EUR
VC102245	Myc-DDK-tagged ORF clone of viral ORF for small virion-associated protein [Infectious bronchitis virus], codon optimized for human cell expression, NP_040834	10 ug	165,00 EUR
VC102246	Myc-DDK-tagged ORF clone of viral ORF for membrane protein [Infectious bronchitis virus], codon optimized for human cell expression, NP_040835	10 ug	330,00 EUR
VC102247	Myc-DDK-tagged ORF clone of viral ORF for 5a protein [Infectious bronchitis virus], codon optimized for human cell expression, NP_040836	10 ug	165,00 EUR
VC102248	Myc-DDK-tagged ORF clone of viral ORF for 5b protein [Infectious bronchitis virus], codon optimized for human cell expression, NP_040837	10 ug	165,00 EUR
VC102249	Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid protein [Infectious bronchitis virus], codon optimized for human cell expression, NP_040838	10 ug	503,00 EUR
VC102251	Myc-DDK-tagged ORF clone for coronavirus nsp8 [Infectious bronchitis virus], codon optimized for human cell expression, NP_740621	10 ug	165,00 EUR
VC102253	Myc-DDK-tagged ORF clone for coronavirus nsp2 (3CL-Pro) [Infectious bronchitis virus], codon optimized for human cell expression, NP_740623	10 ug	330,00 EUR
VC102254	Myc-DDK-tagged ORF clone for coronavirus nsp3 (HD2) [Infectious bronchitis virus], codon optimized for human cell expression, NP_740624	10 ug	330,00 EUR
VC102255	Myc-DDK-tagged ORF clone for coronavirus nsp4 [Infectious bronchitis virus], codon optimized for human cell expression, NP_740625	10 ug	165,00 EUR
VC102256	Myc-DDK-tagged ORF clone for coronavirus nsp5 [Infectious bronchitis virus], codon optimized for human cell expression, NP_740626	10 ug	330,00 EUR
VC102257	Myc-DDK-tagged ORF clone for coronavirus nsp6 [Infectious bronchitis virus], codon optimized for human cell expression, NP_740627	10 ug	165,00 EUR
VC102258	Myc-DDK-tagged ORF clone for coronavirus nsp7 (GLF) [Infectious bronchitis virus], codon optimized for human cell expression, NP_740628	10 ug	165,00 EUR
VC102260	Myc-DDK-tagged ORF clone for coronavirus nsp10 [Infectious bronchitis virus], codon optimized for human cell expression, NP_740630	10 ug	615,00 EUR
VC102261	Myc-DDK-tagged ORF clone for coronavirus nsp11 [Infectious bronchitis virus], codon optimized for human cell expression, NP_740631	10 ug	535,00 EUR
VC102262	Myc-DDK-tagged ORF clone for coronavirus nsp12 [Infectious bronchitis virus], codon optimized for human cell expression, NP_740632	10 ug	503,00 EUR
VC102264	Myc-DDK-tagged ORF clone of viral ORF for leader protein p87 [Infectious bronchitis virus], codon optimized for human cell expression, NP_740634	10 ug	679,00 EUR