Vicia villosa (VVL/VVA) (FITC)

Vicia villosa Lectin (VVL/VVA) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. Fluorescein conjugates display a high rate of photobleaching, signal is sensitive to pH changes, broad fluorescence emission spectrum, and fluorescence quenching on conjugation to biopolymers.

Hairy vetch

≥250ug/ml is usally required to agglutinate type A1 cells.
≤1ug/ml will agglutinate neuraminidase cells.

N-Acetylgalactosamine

N-Acetylgalactosamine

Ca++, Mn++

495nm

515nm

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hairy vetch

Affinity purified

~1mg/ml

Supplied as a liquid in 10mM HEPES, 150mM sodium chloride, 0.05mM CaCl, 0.05mm MnCl, 1% BSA, 0.02% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.