General Info
| Host: | Mouse |
| Applications: | FC |
| Reactivity: | Rat |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: | Mouse monoclonal antibody anti-T-cell surface glycoprotein CD8 alpha chain is suitable for use in Flow Cytometry research applications. |
| Clonality: | Monoclonal |
| Clone ID: | OX-8 |
| Conjugation: | PE |
| Isotype: | IgG1k |
| Formulation: | PBS with 0.05% Proclin300, 1% BSA |
| Storage Instruction: | Recommend storing between 2-8°C and protecting from prolonged exposure to light. Do not freeze this product. |
Information
Description
| Post Translational Modifications | Palmitoylated, but association with CD8B seems to be more important for the enrichment of CD8A in lipid rafts. O-glycosylated. Phosphorylated in cytotoxic T-lymphocytes (CTLs) following activation. |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. |
| Protein Name | T-Cell Surface Glycoprotein Cd8 Alpha ChainCd8 Antigen 32 Kda ChainOx-8 Membrane AntigenCd Antigen Cd8a |
| Database Links | Reactome: R-RNO-198933 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCd8a Localizes To Lipid Rafts Only When Associated With Its Partner Cd8b |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd8 Alpha Chain antibodyAnti-Cd8 Antigen 32 Kda Chain antibodyAnti-Ox-8 Membrane Antigen antibodyAnti-Cd Antigen Cd8a antibodyAnti-Cd8a antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance