Anti-Phospho-PECAM1-Tyr713 antibody (660-740)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:20000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	PECAM1
Gene ID:	5175
Uniprot ID:	PECA1_HUMAN
Immunogen Region:	686-735 aa
Specificity:	Phospho-CD31 (Y713) Polyclonal Antibody detects endogenous levels of CD31 protein only when phosphorylated at Y713.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human PECAM-1 around the phosphorylation site of Tyr713 at the amino acid range 686-735

Post Translational Modifications	Phosphorylated on Ser and Tyr residues after cellular activation by src kinases. Upon activation, phosphorylated on Ser-729 which probably initiates the dissociation of the membrane-interaction segment (residues 709-729) from the cell membrane allowing the sequential phosphorylation of Tyr-713 and Tyr-690. Constitutively phosphorylated on Ser-734 in resting platelets. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation. In endothelial cells Fyn mediates mechanical-force induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.
Function	Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells.PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis. Isoform Delta15: Does not protect against apoptosis.
Protein Name	Platelet Endothelial Cell Adhesion Molecule Pecam-1 Endocam Gpiia' Peca1 Cd Antigen Cd31
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-202733 Reactome: R-HSA-210990 Reactome: R-HSA-216083 Reactome: R-HSA-432142 Reactome: R-HSA-6798695
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Cell Surface Expression On Neutrophils Is Down-Regulated Upon Fmlp Or Cxcl8/Il8-Mediated Stimulation Isoform Long: Cell Membrane Membrane Raft Cell Junction Localizes To The Lateral Border Recycling Compartment (Lbrc) And Recycles From The Lbrc To The Junction In Resting Endothelial Cells Isoform Delta15: Cell Junction
Alternative Antibody Names	Anti-Platelet Endothelial Cell Adhesion Molecule antibody Anti-Pecam-1 antibody Anti-Endocam antibody Anti-Gpiia' antibody Anti-Peca1 antibody Anti-Cd Antigen Cd31 antibody Anti-PECAM1 antibody

Information sourced from Uniprot.org

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