Goat anti-Thrb (zebrafish)
Cat# EB07966
Size : 100ug
Brand : Everest Biotech
| Code | Name | Applications | Availability | Size | Tested species | Grade |
|---|---|---|---|---|---|---|
| EB07966 | Goat Anti-Thrb (zebrafish) Antibody | Pep-ELISA, WB | 100µg specific antibody in 200µl | Zebrafish |
EB07966 (2.5µg/ml) staining of Zebrafish adult Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Target Protein
Principal Names: thrb, thyroid hormone receptor beta, NR1A2, TR-beta, TR[b]1, fj24f03, trb, wu:fj24f03Official Symbol: thrb
Accession Number(s): NP_571415.1
Important Comments: This antibody is not expected to cross-react to Thra.
Immunogen
Peptide with sequence C-DIGSAPIVNAPEGNKVD, from the internal region of the protein sequence according to NP_571415.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 50kDa band observed in Zebrafish adult Heart lysates (calculated MW of 44.3kDa according to NP_571415.1). Recommended concentration: 2-5µg/ml.
Species Reactivity
Tested: ZebrafishExpected from sequence similarity: Zebrafish
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol