Reagents and instruments for immunology, cell biology and molecular biology.
 
> Goat anti-TRPC7

Goat anti-TRPC7

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Cat# EB09257

Size : 100ug

Brand : Everest Biotech


Code Name Applications Availability Size Tested species Grade
EB09257 Goat Anti-TRPC7 Antibody Pep-ELISA, WB, IP Yes 100µg specific antibody in 200µl Chicken, Mouse

Target Protein

Principal Names: TRPC7, transient receptor potential cation channel, subfamily C, member 7, TRP7, likley ortholog of mouse transient receptor potential cation channel, subfamily C, member 7, putative capacitative calcium channel
Official Symbol: TRPC7
Accession Number(s): NP_065122.1; NP_001161049.1; NP_001161048.1; NP_001363830.1
Human GeneID(s): 57113
Non-Human GeneID(s): 26946 (mouse), 282822 (rat)
Important Comments: This antibody is expected to cross-react with isoform 1, 2 and 3 (NP_065122.1; NP_001161049.1; NP_001161048.1).

Immunogen

Peptide with sequence KLCKSKAKSCEND, from the internal region of the protein sequence according to NP_065122.1; NP_001161049.1; NP_001161048.1; NP_001363830.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: A 95kDa band was observed in Chicken Embryo Heart atrial and ventricular myocyte lysates (Sabourin et al,; PMID 21672930). This antibody has been successfully used in WB on Mouse (Murtaza et al.; PMID: 32882106).

Immunoprecipitation: This antibody has been successfully used in IP on lysates of Chicken Embryo Heart (Sabourin et al,; PMID: 21672930).

Species Reactivity

Tested: Chicken, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific References

This antibody has been successfully used in WB on Mouse:
Babar Murtaza, Aziz Hichami, Amira S Khan, Jiri Plesnik, Omar Sery, Alexander Dietrich, Lutz Birnbaumer, Naim A Khan
Implication of TRPC3 channel in gustatory perception of dietary lipids
Acta Physiol (Oxf). 2020 Sep 3;e13554.  doi: 10.1111/apha.13554.
PMID: 32882106

This antibody has been successfully used in WB on Chicken:
Sabourin J, Antigny F, Robin E, Frieden M, Raddatz E.
Activation of Transient Receptor Potential Canonical 3 (TRPC3)-mediated Ca2+ Entry by A1 Adenosine Receptor in Cardiomyocytes Disturbs Atrioventricular Conduction.
J Biol Chem. 2012 Aug 3;287(32):26688-701.
PMID: 22692208

This antibody has been successfully used in WB and IP on Chicken:
Sabourin J, Robin E, Raddatz E.
A key role of TRPC channels in the regulation of electromechanical activity of the developing heart.
Cardiovasc Res. 2011 Jun 13. [Epub ahead of print]
PMID: 21672930