Stemolecule™ hES Cell Cloning & Recovery Supplement
01-0014-500
Brand: Stemolecule™
Stemgent hES Cell Cloning & Recover Supplement is a 1000× concentrate of Thiazovivin (2mM). In culture media it significantly improves the likelihood of primary sub-cloning of pluripotent stem cells from single cells, and increases attachment after passaging. This supplement is a ROCK inhibitor (see Thiazovivin, cat. # 04-0017 for more information).

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Product Information
1,2. This Supplement was validated at a working concentration of 2 μM; however optimal conditions can vary, and they should be determined by the user. When added during a thaw, this Supplement boosts recovery and thawing efficiency. The use of this Supplement also increases attachment after passaging. The hES Cell Cloning and Recovery Supplement is a valuable tool for improving efficiency when working with human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) under stressful conditions.
01-0014-500 Specifications Sheet
Safety Data Sheets:
Protocols:
Lin, T., Ambasudhan, R., Yuan, X., Li, W., Hilcove, S., Abujarour, R., Lin, X., Hahm, H.S., Hao, E., Hayek, A., and Ding S. A chemical platform for improved induction of human iPSCs. Nature Methods 6:805 (2009). Xu, Y., Zhu, X., Hahm, H.S., Wei, W., Hao, E., Hayek, A., and Ding, S. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci USA 107: 8129-8134 (2010).
Additional publications
- Verstegen NNAl Roos FJM; Burka K; Gehartg J; Jager M; de Wolf M; Bijvelds MJC; de Jonge HR; Ardisasmita AI; van Huizen NA; Roest HP; de Jonge J; Koch M; Pampaloni F; Fucha SA; Schene IF; Luider TM; van der Doef HPJ; Bodewes FAJA; de Kleine RHJ; Spee B; Kremers G-J; Clevers HJ; IJzermans JMN; Cuppen E; van der Laan LJW. Human extrahepatic and intrahepatic cholangiocyte organoids show region-specific differentiation potential and model cystic fibrosis-related bile duct disease. Sci Rep 10:21900 (2020).
- Hasaart Karlijn AL; Manders F; van der Hoorn M-L; Verheul M; Poplonski T; Kuijk E; Chuva de Sousa Lopes SM; van Boxtel R. Mutation accumulation and developmental lineages in normal and Down syndrome human fetal haematopoiesis. Sci Reports 10:12991 (2020).
- Sivapatham R; Zeng X.. Generation and characterization of patient-specific induced pluripotent stem cell for disease modeling. Methods Mol Biol 1353:25-44 (2014)