Human UCH-L1/PGP9.5 antibody
Cat# APG0714-100
Size : 100ul
Brand : NKMAX BIO
ICC/IF analysis of PGP9.5 in U87MG cells. The cell was stained with APG0714 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGP9.5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse Brain tissue lysate
Lane 2.: U87MG cell lysate
Lane 1.: Mouse Brain tissue lysate
Lane 2.: U87MG cell lysate
Immunohistochemistry (IHC)
Paraffin embedded sections of human nerve tissue were incubated with anti-human PGP9.5 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.