Matrix Metalloproteinase 9 (MMP-9) BioAssay™ ELISA Kit (Porcine)
Cat# 357391-96T
Size : 96Tests
Brand : US Biological
Shipping Temp
Blue IceStorage Temp
4°C/-20°CThe Matrix Metalloproteinase 9 (MMP-9) BioAssay™ ELISA Kit is a quantitative sandwich assay for the detection of MMP-9 in porcine serum, plasma, tissue homogenates and other biological fluids.
Detection Range:
0.781-50ng/ml
Sensitivity:
0.469ng/ml
Precision:
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Inter-Assay CV: <10%
Assay Principle:
This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate pre-coated with an antibody specific to MMP-9. Standards and samples are added to the appropriate wells, then incubated. Aspirate. Do not wash the plate! The Antibody (Biotin) is added to all the wells. The plate is incubated and then washed. Streptavidin (HRP) is added to each microplate well and incubated then washed. Then the TMB substrate solution is added and incubated. After the TMB substrate solution is added, only those wells that contain MMP-9, the antibody (Biotin) and Streptavidin (HRP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MMP-9 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
Kit Components:
*357391A: Microtiter Strips, 1x48 wells or 1x96 wells
*357391B: Standard, 1x1vial or 2x1 vial
357391C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml
357391D: Antibody (Biotin) (Concentrated), 1x60ul or 1x120ul
357391E: Antibody Dilution Buffer, 1x5ml or 1x10ml
357391F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul
357391G: SABC Dilution Buffer, 1x5ml or 1x10ml
357391H: TMB Substrate, 1x5ml or 1x10ml
357391J: Stop Solution, 1x5ml or 1x10ml
357391K: Wash Buffer, 25X, 1x15ml or 1x30ml
*357391B: Standard, 1x1vial or 2x1 vial
357391C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml
357391D: Antibody (Biotin) (Concentrated), 1x60ul or 1x120ul
357391E: Antibody Dilution Buffer, 1x5ml or 1x10ml
357391F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul
357391G: SABC Dilution Buffer, 1x5ml or 1x10ml
357391H: TMB Substrate, 1x5ml or 1x10ml
357391J: Stop Solution, 1x5ml or 1x10ml
357391K: Wash Buffer, 25X, 1x15ml or 1x30ml
Storage and Stability:
Store unopened *357391A at 4ºC; store at -20ºC once opened. Store unopened *357391B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Summary:
1. Wash plate 2 times before adding standards and samples to wells!
2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times.
3. Add 100ul Antibody (Biotin) working solution to each well and incubate for 60 minutes at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C
6. Aspirate and wash 5 times.
7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
9. Calculate results.
2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times.
3. Add 100ul Antibody (Biotin) working solution to each well and incubate for 60 minutes at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C
6. Aspirate and wash 5 times.
7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
9. Calculate results.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.