Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit

Cat# E-BC-K097-M-96T

Size : 96T

Brand : Elabscience


Detection Principle GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.
Synonyms GSSG;T-GSH
Sample Type Serum,Plasma,Animal tissue,Red blood Cell,cultured Cell
Detection Method Colorimetric method
Detection Instrument Microplate reader (405-415 nm, optimum wavelength: 412 nm)
Research Area Oxidative Stress ,  Cuproptosis ,  Ferroptosis
Other Reagents Required PBS (0.01 mol/L, pH 7.4), Absolute ethanol
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.36 μmol/L
Detection Range 0.36-30 μmol/L
Precision inter-assay CV: 3.9% | intra-assay CV: 0.6%
Assay Time 1 h 40 min
Cat.No. Product Name Sizes
E-BC-K030-M Reduced Glutathione (GSH) Colorimetric Assay Kit 96T , 48T
E-BC-K096-M Glutathione Peroxidase (GSH-Px) Activity Assay Kit 96T , 48T
E-BC-K099-S Glutathione Reductase (GR) Activity Assay Kit 100Assays , 50Assays
E-BC-K278-S Glutathione-S-Transferase (GST) Activity Assay Kit 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human plasma 1
10% Rat liver tissue homogenate 10-20
10% Rat kidney tissue homogenate 1
10% Rat heart tissue homogenate 10-20
10% Mouse brain tissue homogenate 2-5
HepG2 cells 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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331-12036-3
 10x1ml