Reagents and instruments for immunology, cell biology and molecular biology.

Anti-CBX5/HP1-Alpha, biotinylated antibody (Internal) {Biotin}

Print

Cat# NB-22-32853

Size : 100µg

Brand : Neo Biotech

Biotinylated STJ70789 (0. 1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

General Info

Host:	Goat
Applications:	Pep-ELISA/WB
Reactivity:	Human/Mouse/Dog
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Goat polyclonal antibody anti-CBX5/HP1-Alpha, biotinylated (Internal) is suitable for use in ELISA and Western Blot research applications.

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Formulation:	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification:	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration:	0.5 mg/mL
Dilution Range:	WB-0.1-0.3µg/ml ELISA-antibody detection limit dilution 1:128000.
Storage Instruction:	Store at-20°C on receipt and minimise freeze-thaw cycles.

Information

Gene Symbol:	CBX5
Gene ID:	23468
Uniprot ID:	CBX5_HUMAN
Immunogen Region:	Internal
Accession Number:	NP_036249.1
Specificity:	Reported variants represent identical protein: NP_036249.1, NP_001120793.1, NP_001120794.1
Immunogen Sequence:	NKRKSNFSNSADDIK.

Description

Function	Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Protein Name	Chromobox Protein Homolog 5 Antigen P25 Heterochromatin Protein 1 Homolog Alpha Hp1 Alpha
Database Links	Reactome: R-HSA-4551638 Reactome: R-HSA-8953750 Reactome: R-HSA-983231
Cellular Localisation	Nucleus Chromosome Centromere Colocalizes With Hnrnpu In The Nucleus Component Of Centromeric And Pericentromeric Heterochromatin Associates With Chromosomes During Mitosis Associates Specifically With Chromatin During Metaphase And Anaphase Localizes To Sites Of Dna Damage
Alternative Antibody Names	Anti-Chromobox Protein Homolog 5 antibody Anti-Antigen P25 antibody Anti-Heterochromatin Protein 1 Homolog Alpha antibody Anti-Hp1 Alpha antibody Anti-CBX5 antibody Anti-HP1A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance