Reagents and instruments for immunology, cell biology and molecular biology.

Anti-CD41, CD61 (CD41b, GP2B, GP-IIb, GPIIb, GP3A, GP-IIIa, GPIIIa, GTA, HPA3, Integrin alpha 2b, ITGA2B, Integrin beta 3, ITGB3, Platelet Glycoprotein IIb of IIb/IIIa Complex) (HRP) Monoclonal Antibody

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Cat# C2394-35B-HRP-100ul

Size : 100ul

Brand : US Biological

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C2394-35B-HRP CD41, CD61 (CD41b, GP2B, GP-IIb, GPIIb, GP3A, GP-IIIa, GPIIIa, GTA, HPA3, Integrin alpha 2b, ITGA2B, Integrin beta 3, ITGB3, Platelet Glycoprotein IIb of IIb/IIIa Complex) (HRP)

Clone Type

Polyclonal

Host

mouse

Source

chicken

Isotype

IgG1

Grade

Affinity Purified

Applications

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

CD41/61 is a heterodimeric cell surface protein that is expressed on chicken thrombocytes and cells of the thrombocyte lineage. Avian thrombocytes are nucleated equivalents of mammalian platelets that play a role in coagulation and phagocytosis. ||Applications:|Suitable for use in Immunoprecipitation. Other applications not tested. ||Recommended Dilutions:|Optimal dilutions to be determined by the researcher. ||Hybridoma:|X63/A63-653 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: 10B1565 (11C3)|Host: mouse|Source: chicken|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Peripheral blood leukocytes from white leghorn chicken.|Specificity: Recognizes the chicken integrin CD41/61. Reported to induce thrombocyte activation.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Peripheral blood leukocytes from white leghorn chicken.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant.

Specificity

Recognizes the chicken integrin CD41/61. Reported to induce thrombocyte activation.

References

1. Lacoste-Eleaume, A.S. et al. (1994) Biochemical and functional characterization of an Avian homolog of the integrin GPIIb-IIa present on chicken thrombocytes. Exp. Cell. Res. 213: 198 - 209. 2. Corbel, C. et al. (2002) Expression of alpha V beta 3 integrin in the chick embryo aortic endothelium. Int. J. Dev. 46: 827 - 830. 3. Del Cacho, E. et al. (2008) Isolation of chicken follicular dendritic cells. J Immunol Methods. 334: 59-69. 4. Del Cacho, E. et al. (2009) Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses. Vet Immunol Immunopathol. 129: 66-75. 5. Ody, C. et al. (1999) Glycoprotein IIb-IIIa is expressed on avian multilineage hematopoietic progenitor cells. Blood. 93: 2898-906. 6. Chaussé, A.M. et al. (2011) Expression of Toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to Salmonella carrier state. Infect Immun. 79: 3445-54.