Reagents and instruments for immunology, cell biology and molecular biology.

Anti-Ovalbumin (MaxLight 490) Monoclonal Antibody

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Cat# O8075-07A-ML490-100ul

Size : 100ul

Brand : US Biological

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O8075-07A-ML490 Ovalbumin (MaxLight 490)

Clone Type

Polyclonal

Host

mouse

Isotype

IgE

Grade

Purified

Applications

FLISA

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.||Applications: |Suitable for use in FLISA. Other applications not tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Hybridoma: |P3U1 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgE|Clone No: 10F254 (2C6)|Host: mouse|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.|Purity: Purified from tissue culture supernatant.|Immunogen: Ovalbumin.|Specificity: Recognizes ovalbumin (OVA). Suitable for use as a mouse IgE standard in ELISA assays.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Ovalbumin.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.

Purity

Purified from tissue culture supernatant.

Specificity

Recognizes ovalbumin (OVA). Suitable for use as a mouse IgE standard in ELISA assays.

References

1. Stevens, T. et al. (2008) Increased transcription of immune and metabolic pathways in naive and allergic mice exposed to diesel exhaust. Toxicol Sci. 102: 359-70. 2. Fairley,K.J. et al. (2007) Exposure to the immunosuppressant, perfluorooctanoic acid, enhances the murine IgE and airway hyperreactivity response to ovalbumin. Toxicol.Sci. 97: 375-83 3. Ellertsen,L.K. et al. (2010) Maternal allergen immunisation to prevent sensitisation in offspring: Th2-polarising adjuvants are more efficient than a Th1-polarising adjuvant in mice. BMC Immunol. 11: 8-174. Kambayashi, T. et al. (2008) Indirect involvement of allergen-captured mast cells in antigen presentation. Blood. 111:1489-96. 5. Paliwal, S. et al. (2010) One-step acquisition of functional biomolecules from tissues. Proc Natl Acad Sci U S A. 107: 14627-32. 6. Chida, Y. et al. (2007) Early-life psychological stress exacerbates adult mouse asthma via the hypothalamus-pituitary-adrenal axis. Am J Respir Crit Care Med. 175: 316-22. 7. Suzaki, Y. et al. (2007) A small-molecule compound targeting CCR5 and CXCR3 prevents airway hyperresponsiveness and inflammation. Eur Respir J. 31: 783-9. 8. Suzaki, Y. et al. (2005) A potent antiangiogenic factor, endostatin prevents the development of asthma in a murine model. J Allergy Clin Immunol. 116 (6): 1220-7. 9. Hansen, J.S. et al. (2011) Determinants of experimental allergic responses: interactions between allergen dose, sex and age. Scand J Immunol. 73 (6): 554-67. 10. Nygaard, U.C. et al. (2015) Early life exposure to bisphenol A investigated in mouse models of airway allergy, food allergy and oral tolerance. Food Chem Toxicol. 83: 17-25. 11. Shershakova N et al. (2015) Allergen-Specific Immunotherapy with Monomeric Allergoid in a Mouse Model of Atopic Dermatitis. PLoS One. 10 (8): e0135070. 13. Diesner, S.C. et al. (2016) A distinct microbiota composition is associated with protection from food allergy in an oral mouse immunization model. Clin Immunol. pii: S1521-6616(16)30300-X. [Epub ahead of print] 14. Cheung DS et al. (2010) Development of atopy by severe paramyxoviral infection in a mouse model. Ann Allergy Asthma Immunol. 105 (6): 437-443.e1. 15. Garbani, M. et al. (2016) Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice. Allergy. Sep 3. [Epub ahead of print] 16. Mothes, B. et al. (2016) p110y/d Double-Deficiency Induces Eosinophilia and IgE Production but Protects from OVA-Induced Airway Inflammation. PLoS One. 11 (7): e0159310. 18. Andreassen, M. et al. (2015) Cry1Ab protein from Bacillus thuringiensis and MON810 cry1Ab-transgenic maize exerts no adjuvant effect after airway exposure. Scand J Immunol. 81 (3): 192-200. 19. Hamada. K, et al. (2003) Allergen-independent maternal transmission of asthma susceptibility. J. Immunol. 170: 1683-1689.