Reagents and instruments for immunology, cell biology and molecular biology.

Anti-Phospho-IRS1-S307 antibody

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Cat# NB-22-17447-200

Size : 200uL(2x100uL)

Brand : Neo Biotech

Western blot analysis of extracts of mouse brain, using Phospho-IRS1-S307 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
Western blot analysis of various lysates using Phospho-IRS1-S307 (Human Specific) Rabbit polyclonal antibody at 1:1000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37 °C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

General Info

Host:	Rabbit
Applications:	WB
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-IRS1-S307 is suitable for use in Western Blot research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	IRS1
Gene ID:	3667
Uniprot ID:	IRS1_HUMAN
Immunogen:	A synthetic phosphorylated peptide around S307 of human IRS1 (NP_005535.1).
Immunogen Sequence:	TESIT

Description

Post Translational Modifications	Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Phosphorylation of Tyr-896 is required for GRB2-binding. Phosphorylated by ALK. Phosphorylated at Ser-270, Ser-307, Ser-636 and Ser-1101 by RPS6KB1.phosphorylation induces accelerated degradation of IRS1. Phosphorylated on tyrosine residues in response to insulin. In skeletal muscles, dephosphorylated on Tyr-612 by TNS2 under anabolic conditions.dephosphorylation results in the proteasomal degradation of IRS1. Ubiquitinated by the Cul7-RING(FBXW8) complex in a mTOR-dependent manner, leading to its degradation: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2).
Function	May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Protein Name	Insulin Receptor Substrate 1 Irs-1
Database Links	Reactome: R-HSA-109704 Reactome: R-HSA-112399 Reactome: R-HSA-112412 Reactome: R-HSA-1257604 Reactome: R-HSA-1266695 Reactome: R-HSA-198203 Reactome: R-HSA-201556 Reactome: R-HSA-2219530 Reactome: R-HSA-2428928 Reactome: R-HSA-2586552 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-74713 Reactome: R-HSA-74749 Reactome: R-HSA-9603381 Reactome: R-HSA-9725370 Reactome: R-HSA-982772
Alternative Antibody Names	Anti-Insulin Receptor Substrate 1 antibody Anti-Irs-1 antibody Anti-IRS1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance